生物技术通报Issue(7)：134-136,140,4.
c-flag-ago3质粒在人293细胞系中的表达及AGO3蛋白复合物的细胞定位
The Expression of c-flag-ago3 Plasmid in Human 293 Cell Line and the Localization of AGO3 Protein Compounds
刘丽丽 1王荣富1
作者信息
- 1. 安徽农业大学生命科学学院,合肥,230036
- 折叠
摘要
Abstract
It was to transfect c-flag-ago3 plasmid into human 293 cell line to make the c-flag-ago3 stable expression ,and investigate the structure and function of AGO3 protein compounds in human.The methods of affinity tags on the target protein were used ,then the c-flag-ago3 plasmid and the control si-ago3 plasmid which can cause ago3 gene silence were transfect into human 293 cell line, and the effect was observed through the fluorescence microscope. Was observed RT-PCR detected gene content. Flag-ago3 protein was tested by western blot with mouse anti-flag Monoclonal antibody, and located the AGO3 protein compounds in cell. Results indicated that the c-flag-ago3 and the si-ago3 plasmid were inducted successfully in the human 293 cell line and the high expression of the ago3 was comfirmed by immuncytochemical method. It also proved that the AGO3 compounds located in the cytoplasm.关键词
flag标签/c-flag-ago3质粒/人293细胞系/AGO3蛋白复合物Key words
Flag tag c-flag-ago3 plasmid Human 293 cell lines AGO3 protein compounds分类
生物科学引用本文复制引用
刘丽丽,王荣富..c-flag-ago3质粒在人293细胞系中的表达及AGO3蛋白复合物的细胞定位[J].生物技术通报,2009,(7):134-136,140,4.
