中国普通外科杂志2001，Vol.10Issue(2)：134-138,5.
三氧化二砷对肝癌细胞系HLE的影响
Effects of arsenic trioxide on hepatoma cell line HLE
刘连新 1姜洪池 2朱安龙 2周津 3王秀琴 3吴旻3
作者信息
- 1. 哈尔滨医科大学第一临床学院普外科,中国医学科学院肿瘤研究所
- 2. 哈尔滨医科大学第一临床学院普外科,
- 3. 中国医学科学院肿瘤研究所
- 折叠
摘要
Abstract
Objective To observe the effect of various concentration of arsenic trioxide (AT) on hepatoma cell line HLE in variable duration. Methods The cell activity, morphologic changes and apoptosis were studied after being treated with different concentration of AT. Results The effect of AT on hepatoma cell lines was depending on the time and concentration obviously. Hepatoma cells cultured with different concentration of AT presented apoptosis features, such as intact cell membrane, chromatin condesation, nucleic fragmentation and apoptotic body formation; flow cytometry analysis showed that an arrestment at G2/M phase, an apoptosis peak presented before sub-G1 cell peak, and a mark DNA ladder of apoptosis appeared. Conclusions AT can obviously inhibit the growth of hepatoma cell through inducing hepatoma cell apoptosis.关键词
三氧化二砷/药理学/肿瘤细胞,培养的/药物作用/细胞系,转化/药物作用/细胞凋亡/癌,肝细胞分类
医药卫生引用本文复制引用
刘连新,姜洪池,朱安龙,周津,王秀琴,吴旻..三氧化二砷对肝癌细胞系HLE的影响[J].中国普通外科杂志,2001,10(2):134-138,5.
