首页|期刊导航|中华医学杂志（英文版）|Hydrogen sulfide facilitates carotid sinus baroreceptor activity in anesthetized male rats
中华医学杂志（英文版）2007，Vol.120Issue(15)：1343-1347,5.
Hydrogen sulfide facilitates carotid sinus baroreceptor activity in anesthetized male rats
Hydrogen sulfide facilitates carotid sinus baroreceptor activity in anesthetized male rats
摘要
Abstract
Background It has been reported that hydrogen sulfide (H2S) could relax vascular smooth muscle by direct activation of KATP channels and hyperpolarization of the membrane potential. Recently, our study has shown that H2S facilitated carotid baroreflex. This study was conducted to investigate the effect of H2S on carotid baroreceptor activity (CBA).Methods The functional curve of carotid baroreceptor (FCCB) was constructed and the functional parameters of carotid baroreceptor were measured by recording sinus nerve afferent discharge in anesthetized male rats with perfused isolated carotid sinus.Results H2S (derived from NarHS) 25, 50 and 100 μmol/L facilitated CBA, which shifted FCCB to the left and upward.There was a marked increase in peak slope (PS) and peak integral value of carotid sinus nerve charge (PIV) in a concentration-dependent manner. Pretreatment with glibenclamide (20 μmol/L), a KATP channel blocker, the above effects of H2S on CBA were abolished. Pretreatment with Bay K8644 (an agonist of calcium channels, 500 nmol/L) eliminated the role of H2S on CBA. An inhibitor of cystathionine Y-lyase (CSE), DL-propargylglycine (PPG, 200 μmol/L) inhibited CBA in male rats and shifted FCCB to the right and downward.Conclusions Our results suggest that exogenous H2S exerts a facilitatory role on isolated CBA through opening KATP channels and further closing the calcium channels in vascular smooth muscle. Endogenous H2S may activate CBA in vivo.关键词
hydrogen sulfide/potassium channels/glibenclamide/baroreceptors/Bay K8644Key words
hydrogen sulfide/potassium channels/glibenclamide/baroreceptors/Bay K8644分类
医药卫生引用本文复制引用
XIAO Lin,WU Yu-ming,WANG Ru,LIU Yi-xian,WANG Fu-wei,HE Rui-rong..Hydrogen sulfide facilitates carotid sinus baroreceptor activity in anesthetized male rats[J].中华医学杂志（英文版）,2007,120(15):1343-1347,5.基金项目
The study was supported by the Natural Science Foundation of Hebei Province of China (No. C2007000821). （No. C2007000821）
