中华医学杂志（英文版）2006，Vol.119Issue(16)：1353-1358,6.
A novel full-length gene of human ribosomal protein L14.22 related to human glioma
A novel full-length gene of human ribosomal protein L14.22 related to human glioma
QI Zhen-yu 1HUI Guo-zhen 1LI Yao 2ZHOU Zong-xiang 2GU Shao-hua 2XIE Yi2
作者信息
- 1. Department of Neurosurgery, First Affiliated Hospital of Soochow University, Suzhou 215006, China
- 2. State Key Laboratory of Genetic Engineering, Institute of Genetics,Fudan University, Shanghai 200433, China
- 折叠
摘要
Abstract
Background This study was undertaken to obtain differentially expressed genes related to human glioma by cDNA microarray and the characterization of a novel full-length gene. Methods Total RNA was extracted from human glioma and normal brain tissues, and mRNA was used as a probe. The results of hybridization procedure were scanned with the computer system. The gene named 507E08clone was subsequently analyzed by northern blot, bioinformatic approach, and protein expression.Results Fifteen differentially expressed genes were obtained from human glioma by hybridization and scanning for four times. Northern blot analysis confirmed that the 507E08 clone was low expressed in human brain tissue and over expressed in human glioma tissues. The analysis of BLASTn and BLASTx showed that the 507E08clone was a novel full-length gene, which codes 203 amino acid of protein and is called human ribosomal protein 14.22 gene. The nucleotide sequence had been submitted to the GenBankTM with the accession number of AF329277. After expression in E. Coli., protein yielded a major band of apparent molecular mass 22 kDa on an SDS-PAGE gel.Conclusions cDNA microarray technology can be successfully used to identify differentially expressed genes.The novel full-length gene of human ribosomal protein 14.22 may be correlated with the development of human glioma.关键词
glioma/ribosomal proteins/genesKey words
glioma/ribosomal proteins/genes分类
医药卫生引用本文复制引用
QI Zhen-yu,HUI Guo-zhen,LI Yao,ZHOU Zong-xiang,GU Shao-hua ,XIE Yi..A novel full-length gene of human ribosomal protein L14.22 related to human glioma[J].中华医学杂志（英文版）,2006,119(16):1353-1358,6.
