华西医科大学学报2001，Vol.32Issue(1)：136-139,4.
聚合酶链反应-微孔板杂交法检测结核杆菌的临床应用及其评价
Clinical Application and Evaluation of Polymerase Chain Reaction-Mic rowell Plate Hybridization Assay in the Detection of Mycobacterium tuberculosis
杨正林 1傅明德 2杜琼 3杨明清 1乔国蓉 4唐蓉4
作者信息
- 1. 四川省人民医院 检验科
- 2. 华西医科大学基础医学院生物化学教研室
- 3. 四川省人民医院检验科
- 4. 成都铁路局结核病医院
- 折叠
摘要
Abstract
Objective To evaluate the clinical application an d feasibilityof polymerase chain reaction-microwell plate hybridization assay in the detection of M ycobacterium tuberculosis. Methods 1130 specimens with strong s uspicion for myc obacterium tuberculosis were collected from the hospitals and were detected by fast bacilli stain,culture,PCR-electrophoresis and PCR-microwell plate hybridi za tion respectively. The laboratory results were analyzed in combination with the symptoms and signs of patients and the observations on treatment.Also detected were 100 samples from the clinically evidenced non-tuberculosis patients. Results In the 100 samples collected from the patients without tuber culosis, the PCR- h ybridization method and culture method did not detect Mycobacterium tuberculosis , but the fast bacilli stain method and PCR-electrophoresis method brought out o ne and two false-positive results respectively. The results of testing the 1030 clinical samples which probably contained Mycobacterium tuberculosis demonstrate d that the PCR-hybridization method had the highest positive rate (481/1030) am o ng the four methods, and the positive rates of the other three methods were PCR - electrophoresis (406/1030)， culture(365/1030) and fast bacilli stain(256/1030) i n proper order. The chi-square test showed that there were significant differen c e between the PCR-hybridization method and the other three methods respectively (P<0.0083 or P<0.0017). Conclusion PCR-Hybridization m e thod is specific, sensitive,accurate and fast in detecting mycobacterium tubercu losis; it is worthy to be used clinically.关键词
聚合酶链反应-微孔板杂交/结核杆菌/应用与评价分类
医药卫生引用本文复制引用
杨正林,傅明德,杜琼,杨明清,乔国蓉,唐蓉..聚合酶链反应-微孔板杂交法检测结核杆菌的临床应用及其评价[J].华西医科大学学报,2001,32(1):136-139,4.
