环境工程学报2009，Vol.3Issue(8)：1365-1370,6.
DGGE、T-RFLP、LH-PCR对两种活性污泥的微生物种群多样性分析的比较
Comparison of DGGE,T-RFLP and LH-PCR in the analysis of microbial community diversity for two kinds of activated sludges
摘要
Abstract
Using three PCR-based molecular ecological techniques,i.e.DGGE,T-RFLP and LH-PCR,the microbial community diversity of activated sludges from two typical wastewater treatment systems(domestic and coking)was investigated.The advantages and disadvantages of the three techniques were compared and the consideration of method selection under different conditions was suggested.DGGE displayed many bands,nevertheless.the sources of error were multiple.T-RFLP was sensitive,but it required suitable endonuclease and reaction condition,and the result from the T-RFLP phylogenetic assignment tool(PAT)was uncertainty to some extent.LH-PCR was characterized by simple operation and stable result.Under the existing technical level.it was still unable to determine the accuracy of the three techniques.However.LH-PCR had displayed a certain potential in the analysis of microbial community diversity for activated sludges.关键词
DGGE/T-RFLP/LH-PCR/活性污泥/微生物种群Key words
DGGE/T-RFLP/LH-PCR/activated sludge/microbial community分类
资源环境引用本文复制引用
孙庆华,柏耀辉,赵翠,温东辉,唐孝炎..DGGE、T-RFLP、LH-PCR对两种活性污泥的微生物种群多样性分析的比较[J].环境工程学报,2009,3(8):1365-1370,6.基金项目
国家高技术研究发展计划(863)项目(2006AA062336) （863）
国家自然科学基金资助项目(50878001) （50878001）
