中国药科大学学报2006，Vol.37Issue(2)：137-141,5.
手性药物佐米曲普坦的磺化β-环糊精毛细管电泳分析
Enantiomeric separation of zolmitriptan by CE with a sulfated β-cyclodextrin chiral selector
吴春勇 1狄斌 1姚晓敏 2杨静 1刘文英1
作者信息
- 1. 中国药科大学药物分析学教研室,南京,210009
- 2. 南京海辰药业有限公司,南京,210046
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摘要
Abstract
Aim:To develop a practical chiral CE method for the quantitative determination of the unwanted enantiomer[( R )-enantiomer]presented in zolmitriptan. Methods:The background electrolyte was 20 mmol/L sodium dihydrogenphosphate solution with 1% S-β-CD,adjusted to pH 3.50 with phosphoric acid. A fused-silica capillary(60 cm×50 μm ID,effective length 51.5 cm)was used at 20 ℃ for the separation. The applied voltage was -30 kV. The samples were loaded by hydrodynamic injection(50 mbar pressure,6 s). UV was measured at 220 nm. Results:Zolmitriptan and its chiral impurity were baseline resolved ( R s=6.66). The linearity was good over the concentration range from 4 to 80 μg/mL( r =0.999 8) of ( R )-enantiomer. The injection precision (expressed as CV%) was 2.83%. The average recovery was 99.97%( n =9). The limit of detection was 1.5 μg/mL. The host-guest complex binding constants were 964 and 905 mol-1 for ( R )-enantiomer and zolmitriptan,respectively. Conclusion:The method is suitable for the determination of ( R )-enantiomer in zolmitriptan and binding constants of zolmitriptan enantiomers to S-β-CD.关键词
毛细管电泳/手性分离/佐米曲普坦/磺化β-环糊精/结合常数Key words
CE/enantiomeric separation/zolmitriptan/sulfated β-cyclodextrin/binding constants分类
化学化工引用本文复制引用
吴春勇,狄斌,姚晓敏,杨静,刘文英..手性药物佐米曲普坦的磺化β-环糊精毛细管电泳分析[J].中国药科大学学报,2006,37(2):137-141,5.
