四川大学学报（自然科学版）2006，Vol.43Issue(6)：1374-1378,5.
麦冬道地性的RAPD分析
Identification of Ophiopogon japonicus (L. f. ) Ker-Gawl. as Geo-herbal Based on RAPD Analysis
雷高鹏 1乔代蓉 1熊焰 1李世林 1徐柯 1曹毅1
作者信息
- 1. 生物资源与生态环境教育部重点实验室,四川大学生命科学学院,成都,610064
- 折叠
摘要
Abstract
To identify Ophiopogon japonicus (L. f. ) Ker-Gawl. as geo-herbal, 13 populations of O. japonicus (L. f. ) Ker-Gawl. and two other species, Ophiopogon bodinieri L évl and Ophiopogon bockianus Diels, were investigated using the random amplified polymorphic DNA (RAPD) method. All natural populations of O. japonicus (L. f. ) Ker-Gawl. from Sichuan, Hubei, Zhejiang, Shanghai, and two other species belong to the same genus Ophiopogon. Eight RAPD primers selected from 40 random decamer oligonucleotides were used for the analysis. Five hundred and four RAPD bands were generated by the 8 selected primers and the percentage of polymorphic bands was 89.4% on average. The 13 populations of O. japonicus (L. f. ) Ker-Gawl.and two other species were clustered in unweighted pair group method with arithmetic average (UPGMA) tree. The largest distance was found between O. bockianus Diels, O. bodinieri Lévl.and the populations of O. japonicus (L. f. ) Ker-Gawl.. Among the O. japonicus (L. f. ) Ker-Gawl., the largest distance was found between big leaf type population collected from Sichuan University and the other populations. A high level of genetic variation occurred among the different clime and leaf-type populations. The authors supported that RAPD analysis distinguish O. japonicus (L. f. ) Ker-Gawl. from other species of genus Ophiopogon and identify the population of O.japonicus (L. f. ) Ker-Gawl. used as geo-herbal.关键词
麦冬/RAPD/道地中药Key words
Ophiopogon japonicus (L. F. ) Ker-Gawl./RAPD/geo-herbal分类
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雷高鹏,乔代蓉,熊焰,李世林,徐柯,曹毅..麦冬道地性的RAPD分析[J].四川大学学报（自然科学版）,2006,43(6):1374-1378,5.
