中国现代医学杂志2007，Vol.17Issue(2)：138-141,4.
内皮素对Oddi括约肌功能影响的实验研究
Study on the effect of endothelin-1 on the contraction of Oddi sphincter
摘要
Abstract
[Objective] To investigate the direct effect of endothelin-1 on the contraction of Oddi sphincter, the signal transduction pathway was also explored. [Methods] Accumulated ET-1 were given in K-H solution to made a final concentration (0.1nM～100nM), the contraction amplitude and frequency of Oddi sphincter were observed,Anti-ET serum, Nicardipine, BQ-123 were added to detect their effects on the contraction of Oddi sphincter, and PKC activities in Oddi sphincter were also detected. [Results] ET addition resulted in the contraction of resting Oddi sphincter, and the contraction intensity increased with the rising ET concentration. No obvious inhibition effect on Oddi sphincter contraction was induced by Nicardipine, while anti-ET serum and ET receptor antagonist reduced the Oddi sphincter contraction significantly. The PKC activity in cytoplasm of Oddi sphincter enhanced with the rising ET-1 concentration, and the contraction of Oddi sphincter increased at the same time. [Conclusion] ET-1 addition results in the significant contraction of the ex vivo Oddi sphincter, the contraction and ET concentration are dose-effect related. Anti-ET serum and ET receptor antagonists strongly offset the function of ET. PKC is involved in the signal transduction pathway of the Oddi sphincter contraction induced by ET-1.关键词
Oddi括约肌/内皮素/内皮素受体Key words
Oddi sphincter/endothelin/endothelin receptor分类
医药卫生引用本文复制引用
葛春林,罗晓光,李智,孙树..内皮素对Oddi括约肌功能影响的实验研究[J].中国现代医学杂志,2007,17(2):138-141,4.基金项目
Founded by school Scientific Research Item of Liaoning Department of Education(202013616) （202013616）
