中华医学杂志（英文版）2003，Vol.116Issue(9)：1382-1385,4.
Shenfu injection reduces toxicity of bupivacaine in rats
Shenfu injection reduces toxicity of bupivacaine in rats
摘要
Abstract
Objective To investigate the effects of injecting Shenfu, an extract of traditional Chinese herbal medicines, on the central nervous system (CNS) and the cardiac toxicity of bupivacaine in rats. Methods Sixteen male Sprague-Dawley rats, weighing form 280 to 320 g, were randomly assigned to two groups (n=8 in each group). Animals in the control group received a saline injection 10 ml/kg while animals in the Shenfu group received an injection of Shenfu 10 ml/kg intraperitoneally 30 minutes before intravenous infusion of bupivacaine. Lead Ⅱ of an electrocardiogram (EEG) was continuously monitored after 3 needles were inserted into the skin of both forelimbs and the left hind-leg of each rat. The femoral artery was cannulated for measurement of arterial blood pressure and blood sampling. The femoral vein was cannulated for the infusion of bupivacaine. After baseline measurement (arterial blood pressure, heart rate and arterial blood gas), 0.5% bupivacaine was infused intravenously at a rate of 2 mg*kg-1*min-1 to all animals until asystole occurred. The time of bupivacaine-induced convulsions, arrhythmia and asystole were determined. The dose of bupivacaine was then calculated at the corresponding time point. Results The doses of bupivacaine that induced convulsions, arrhythmia and cardiac arrest were remarkably larger in Shenfu injection-treated animals than in saline-treated rats ［convulsions, (10.5±1.9) mg/kg vs (7.2±1.5) mg/kg; arrhythmia (10.5±2.0) mg/kg vs (7.2±1.9) mg/kg; asystole, (32.8±8.5) mg/kg vs (25.0±5.0) mg/kg; P=0.006, 0.009 and 0.044, respectively］. Conclusion The Shenfu injection is able to reduce the toxicity of bupiralaine to CNS and cardiac system in rats.关键词
bupivacaine/Shenfu injection/toxicityKey words
bupivacaine/Shenfu injection/toxicity分类
医药卫生引用本文复制引用
王强,刘艳红,雷毅,杨静,陈绍洋,陈敏,熊利泽..Shenfu injection reduces toxicity of bupivacaine in rats[J].中华医学杂志（英文版）,2003,116(9):1382-1385,4.基金项目
This study was supported by a grant from the "Golden Bridge" project of Xijing Hospital (No. 004). （No. 004）
