首页|期刊导航|中国农业科学（英文版）|Construction of SMART cDNA Library of Sheep Ovary and Identification of Candidate Gene by Homologous Cloning

Construction of SMART cDNA Library of Sheep Ovary and Identification of Candidate Gene by Homologous Cloning

中国农业科学（英文版）2007，Vol.6Issue(11)：1390-1395,6.

Construction of SMART cDNA Library of Sheep Ovary and Identification of Candidate Gene by Homologous Cloning

作者信息

1. National Center for Molecular Genetics and Breeding of Animal/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, P.R. China;Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture/Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266007, P.R. China;Center for Experimental Animal, Shandong Academy of Medical Sciences, Jinan 250001, P.R. China;National Center for Molecular Genetics and Breeding of Animal/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, P.R. China;National Center for Molecular Genetics and Breeding of Animal/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, P.R. China;National Center for Molecular Genetics and Breeding of Animal/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, P.R. China;National Center for Molecular Genetics and Breeding of Animal/Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing 100094, P.R. China
折叠

摘要

Abstract

The cDNA library of an ovary from Small Tail Han sheep before estrus was constructed by switching mechanism at 5' end of RNA transcript (SMART) approach. This library had a plaque titer of 1 × 109pfu mL-1 and a 96% recombinant ratio of which the fragment length of inserted average eDNA sequences was 1.0 kb. Based on bioinformatics analysis of the sequences, we obtained 338 expressed sequence tags (ESTs) from 380 cDNA clones which indicated 191 contigs. These contigs consist of 89 unmatched ESTs, 9 homologous known genes in sheep, and 93 homologous sequences in species of mouse, bovine, and human beings, including 19 sequences expressed in the ovary or follicle and 14 unknown sequences.Several candidate genes associated with sheep reproduction trait such as epidermal growth factor (EGF), estrogen receptor (ESR), Inhibin, follicle stimulating hormone receptor (FSHR), prostaglandin (PG), and transforming growth factor-β (TGF-β) were identified and the homologous were cloned from this library, which will contribute to compile expression profies and find the major genes of prolificacy of Small Tail Han sheep.

关键词

candidate gene, EST, ovary, Small Tail Han sheep, SMART cDNA library

Key words

candidate gene, EST, ovary, Small Tail Han sheep, SMART cDNA library

分类

农业科技

引用本文复制引用

..Construction of SMART cDNA Library of Sheep Ovary and Identification of Candidate Gene by Homologous Cloning[J].中国农业科学（英文版）,2007,6(11):1390-1395,6.

基金项目

This research was supported by the National Natural Science Foundation of China (30270949). （30270949）

中国农业科学（英文版）

ISSN：2095-3119

访问量0

下载量0

段落导航