中国普通外科杂志2001，Vol.10Issue(2)：139-141,3.
γ-干扰素对肝癌细胞株Hep-G2 Fas,Bcl-2表达的影响
Effect of γ-interferon on the expresson of Fas, Bcl-2 on Hep-G2 hepatocellular carcinoma cells
郑岩松 1林永堃 1石铮 1吕新生2
作者信息
- 1. 福建医科大学附属第一医院普外科,
- 2. 中南大学湘雅医院普外科,
- 折叠
摘要
Abstract
Objective To investigate the influence of γ-interferon (γ-IFN) on liver cancer cell line (Hep-G2). Methods Observing the expression of Fas and Bcl-2 by γ-IFN-pretreated Hep-G2 cells via immunohistochemical stain; subsequently treating these cells with adrimysin, and observing the cell death rate and apoptosis of these cell by MTT and electroscopy. Results (1) γ-IFN up-regulating the expression of Fas protein and down-regulating Bcl-2 protein (P<0.05), and the sensitivity of pretreated hep-G2 cell to adrimysin was increased. Conclusions γ-IFN can rise the sensitivity of Hep-G2 to adrimysin via regulating the expression of Fas and Bcl-2.关键词
γ-干扰素/药理学/基因表达/药物作用/基因,FAS/基因,Bcl-2/
肿瘤细胞,培养的/药物作用/细胞系,转化/药物作用/细胞凋亡/癌,肝细胞分类
医药卫生引用本文复制引用
郑岩松,林永堃,石铮,吕新生..γ-干扰素对肝癌细胞株Hep-G2 Fas,Bcl-2表达的影响[J].中国普通外科杂志,2001,10(2):139-141,3.
