南方医科大学学报2006，Vol.26Issue(2)：139-143,5.
链霉亲合素-DTPA-Gd经腹腔和静脉注射后对荷瘤裸鼠磁共振肿瘤靶向成像的强化效应
Targeted magnetic resonance imaging with intraperitoneal and intravenous streptavidin (SA)-DTPA-Gd: a comparative study in tumor bearing nude mice
摘要
Abstract
Objective To investigate the effect of streptavidin (SA)-DTPA-Gd after intraperitoneal and intravenous administration for tumor enhancement in targeted magnetic resonance imaging (MRI). Methods Biotinylated monoclonal antibody CL3 (600 μg) was intravenously injected into 12 BALB/c nude mice with subcutaneous inoculation of LoVo cells, followed by administration of 80 μg avidin as the chaser 24 h later and then SA-DTPA-Gd was injected intravenously or intraperitoneally after another 30 min. MRI was performed before and 20, 60 min and 3, 6, 9, 12 h after the injection of the contrast agents, and the MR signal intensity of the tumor and liver was determined. Result The maximum enhancement ratio of the tumor was 70.2%in the intravenous injection group and 46.4% in the intraperitoneal group, showing significant difference between them. The maximal enhancement rate of the liver was 23.7% in the intraperitoneal group and 20.4% in the intravenous group, showing no significant difference. Conclusion MR targeted imaging with biotinylated monoclonal antibody CL3 and SA-DTPA-Gd has specific enhancement effect. Higher blood level of SA-DTPA-Gd in the intravenous group facilitates the tumor enhancement in MRI in subcutaneous tumor model.关键词
链霉亲合素/磁共振成像/单克隆抗体Key words
streptavidin/magnetic resonance imaging/monoclonal antibody分类
医药卫生引用本文复制引用
叶靖,许乙凯,刘岘,吕国士..链霉亲合素-DTPA-Gd经腹腔和静脉注射后对荷瘤裸鼠磁共振肿瘤靶向成像的强化效应[J].南方医科大学学报,2006,26(2):139-143,5.基金项目
Supported by National Natural Science Foundation of China (30371608)and Natural Science Foundation of Guangdong Province (020053) （30371608）
