中华医学杂志（英文版）2003，Vol.116Issue(9)：1399-1403,5.
Extracellular matrix synthesis and ultrastructural changes of degenerative disc cells transfected by Ad/CMV-hTGF-β1
Extracellular matrix synthesis and ultrastructural changes of degenerative disc cells transfected by Ad/CMV-hTGF-β1
谭延斌 1胡有谷 1谭江威1
作者信息
- 1. Department of Orthopedic Surgery, Affiliated Hospital of Medical College, Qingdao University, Qingdao 266003, China
- 折叠
摘要
Abstract
Objective To determine whether the synthesis of proteoglycan, collagen and associated ultrastructure are related to the adenovirus-mediated gene transferred to adult degenerative cells.Methods Adenovirus/cytomegalovirus human transforming growth fector-β1 (Ad/CMV-hTGF-β1) was used to transfect degenerative cells. Antonopulos method, Miamine method and transmission electrion microscopy were conducted to study the synthesis of proteoglycan, collagen, and ultrastructure, respectively. Cell cultures were established from the nucleus pulpous and annulus fibrosus tissues, which were taken from surgery.Results Nucleus pulpous and annulus fibrosus cells were efficiently transduced by the adenoviral vector carrying hTGF-β1 gene. The synthesis of proteoglycan and collagen increased compared with the control group (P<0.05). The metabolism of cells was slightly improved. No significant toxic effects were found.Conclusions Expression of hTGF-β1 gene is efficient to accelerates proteoglycan synthesis and thus accelerates the improvement of collagen. The function and structure of degenerative cells are improved. Ad/CMV-hTGF-β1 may be suitable for treating disc degeneration. 关键词
degeneration/growth factor/proteoglycan/collagen/ultrastructure/gene therapy/adenovirusKey words
degeneration/growth factor/proteoglycan/collagen/ultrastructure/gene therapy/adenovirus分类
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谭延斌,胡有谷,谭江威..Extracellular matrix synthesis and ultrastructural changes of degenerative disc cells transfected by Ad/CMV-hTGF-β1[J].中华医学杂志（英文版）,2003,116(9):1399-1403,5.
