中国现代医学杂志2004，Vol.14Issue(10)：1-4,4.
大鼠局灶性脑缺血再灌注后神经细胞凋亡和Bcl-2蛋白表达的改变及银杏叶提取物的保护作用
Effects of Ginkgo biloba extract on apoptosis and expression of Bcl- 2 in rats following focal cerebral ischemia and reperfusion
张鸿 1毕国荣 1赵冬雪 1郑东明1
作者信息
- 1. 中国医科大学第二附属医院神经内科,辽宁,沈阳,110004
- 折叠
摘要
Abstract
Objective: To study the effects of Ginkgo biloba extract (GBE) on apoptosis and the expression of Bcl - 2 following focal cerebral ischemia and reperfusion. Methods: The model of middle cerebral artery occlusion(MCAO) and reperfusion was set up. Forty Wistar male rats were randomly divided into 4 groups: A ( sham - opera-ted group ), B (ischemic group ), C (small -dose GBE treatment group) and D (high -dose GBE treatment group). GBE of 20 mg/kg and 40 rmg/kg was respectively intraperitoneally injected to rats 0.5 h before and 1 h after focal cerebral ischemia. The brain sections were used for POD staining and Bcl -2 immunohistochemical staining.Meanwhile, the sections were stained by TIC and HE for surveying the infarct volume and the degree of ischemia. Re-sults:The infarct volumes and apoptotic cells of groups C and D were significantly less than group B (P ＜0.01 ),those of group D were less than group C(P ＜ 0.05 ). The expression of Bcl- 2 positive cells in groups C and D was significantly higher than that in group B (P ＜ 0.01 ), that in group D was higher than that in group C ( P ＜ 0.05 ).Conclusions: GBE can decrease the number of apoptotic cells after ischemia and reperfusion by up - regulating Bcl -2. The effect of GBE is correlated with dose.关键词
银杏叶提取物/凋亡/Bcl-2/脑缺血再灌注Key words
Ginkgo biloba extract/apoptosis/Bcl- 2/cerebral ischemia and reperfusion分类
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张鸿,毕国荣,赵冬雪,郑东明..大鼠局灶性脑缺血再灌注后神经细胞凋亡和Bcl-2蛋白表达的改变及银杏叶提取物的保护作用[J].中国现代医学杂志,2004,14(10):1-4,4.
