中国林学(英文版)2002，Vol.4Issue(1)：1-4,4.
胡杨悬浮细胞原生质体游离及质膜单离子通道测定
Isolation of Protoplast and Ion Channel Recording in Plasma Membrane of Suspension Cells of Populus euphratica
摘要
Abstract
The authors used suspension cells of Populus euphratica to isolate protoplast in the present study. Protoplasts were successfully obtained after 4 hours incubation in enzyme solution containing 1.0 % cellulase "onozuka" R-10, 0.01% pectolyase Y-23,0.15% macerozyme R-10 and 0.1% hemicellulase at 25℃. Outward and inward single channels in plasma membrane were observed using cell-attached recording of patch-clamp technique.In this study, single channel records showed that more than one species of channel were obtained. These attempts in protoplast isolation and ion channel recording offers the opportunity to characterize cellular mechanisms of salt tolerance in tree species.关键词
胡杨/悬浮细胞/原生质体/膜片钳/细胞贴附技术/单通道Key words
Populus euphratica/suspension cells/protoplasts/patch-clamp/cell-attached recording tech-nique/single ion channel分类
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..胡杨悬浮细胞原生质体游离及质膜单离子通道测定[J].中国林学(英文版),2002,4(1):1-4,4.基金项目
Supported in part by the National Natural Science Foundation of China(NNSF) (Grant No. 30070613 ), the Foundation for the Authors of National Excellent Doctoral Dissertation of P. R. China( Grant No. 200152), National Laboratory of Biomembranes and Membra （NNSF）
