中国林学（英文版）2005，Vol.7Issue(4)：1-4,4.
Identification and Characterization of Peptides Binding AgEG1 from a Phage Display Library
Identification and Characterization of Peptides Binding AgEG1 from a Phage Display Library
摘要
Abstract
Endoglucanases are the main cellulolytic enzymes of Anoplophora glabripennis. Their high activities in cellulose digestion as well as its good kinetic properties make it an attractive target for development of cellulase inhibitors. In this study, random peptide phage display technology was employed to identify peptides that bound the AgEG1, a member of endoglucanase isozymes. Phage clones with peptide LPPNPTK and XPP (X is residue T, L, A or H) motif frequently occurred in the selected phage population and showed a higher phage recovery than other clones. Peptide LPPNPTK was chemically synthesized and characterized for its binding activities to AgEG1. The synthetic peptide exhibited high specificity for AgEG1. The peptide LPPNPTK has the potential to be developed into inhibitors of the endoglucanase of A. Glabripennis.关键词
larvae ofAnoplophora glabripennis, random peptide phage display library, AgEG1, synthetic peptideKey words
larvae ofAnoplophora glabripennis, random peptide phage display library, AgEG1, synthetic peptide分类
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..Identification and Characterization of Peptides Binding AgEG1 from a Phage Display Library[J].中国林学（英文版）,2005,7(4):1-4,4.基金项目
Supported by the National Natural Science Foundation of China (Grant No. 39900116) （Grant No. 39900116）
