中国实用神经疾病杂志2009，Vol.12Issue(7)：1-4,4.
黄体酮对活化小胶质细胞TNF-α与IL-1β分泌的影响
Progesterone inhibits the expression of tumor necrosis factor-alpha and interleukin-1β in cultured microglia
王建平 1王伟 2蒋超1
作者信息
- 1. Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
- 2. Department of Neurology, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, China
- 折叠
摘要
Abstract
Objective Inflammatory cytokines play important roles in the pathophysiology of cerebral infarction and other central nervous system diseases.This study was designed to investigate the influence of progesterone on lipopolysaccharide-induced expression of tumour necrosis factor-alpha (TNF-α) and interleukin-1β (IL-1β) in primary cultured microglia.Methods Microglia were obtained from cerebral cortexes of neonatal Sprague Dawley rats.Microglia were separated,purificated, cultured and activated.ELISA was used to detect the level of TNF-α, IL-1β in supernate fluid before and after induced with lipopolysaccharide (LPS) or influenced by progesterone.Results LPS strongly induced the expression of TNF-α, IL-1β in microglia from cerebral cortexes.Progesterone inhibited the expression of TNF-α, IL-1β.Conclusion progesterone significantly reduced the expression of inflammatory factors generated by microglia and inhibited the activation of microglia in vitro.关键词
Progesterone/Microglia/Activation/Inflammatory cytokinesKey words
Progesterone/Microglia/Activation/Inflammatory cytokines分类
医药卫生引用本文复制引用
王建平,王伟,蒋超..黄体酮对活化小胶质细胞TNF-α与IL-1β分泌的影响[J].中国实用神经疾病杂志,2009,12(7):1-4,4.
