首页|期刊导航|南京医科大学学报（英文版）|Effects of Glucagon-Like Peptide 1 on PDX-1, PAX-6 and NKx2.2 Gene Expressions in Isolated Pancreatic Islets
南京医科大学学报（英文版）2006，Vol.20Issue(3)：141-144,4.
Effects of Glucagon-Like Peptide 1 on PDX-1, PAX-6 and NKx2.2 Gene Expressions in Isolated Pancreatic Islets
Effects of Glucagon-Like Peptide 1 on PDX-1, PAX-6 and NKx2.2 Gene Expressions in Isolated Pancreatic Islets
摘要
Abstract
Objective: To observe the effect of glucagon-like peptide 1 (GLP-1) on the gene expressions of transcription factors (PDX-1, PAX-6 and NKx2.2) in freshly isolated rat pancreatic islets and investigate the associated physiological and therapeutic implication of GLP-1. Methods: The isolated rat islets were incubated with 10 nmol/L GLP-1 for 1, 3 and 5 days, respectively. Total cellular RNA was extracted and the expressions of PDX-1, PAX-6 and NKx2.2 gene were detected by semiquantity RT-PCR. Results: Compared with the control group, the PDX-1, PAX-6 and NKx2.2 gene expressions were significantly increased after co-cultured with GLP-1 for 1 day (P ＜ 0.05). The effect was shown in a time-dependent manner. All three gene expressions reached the peak on the 5th day. Conclusion: GLP-1 can improve the function of pancreatic islet by regulating the gene expressions of transcription factors in β cells.关键词
glucagon-like peptide 1/islet/transcription factor/diabetes mellitusKey words
glucagon-like peptide 1/islet/transcription factor/diabetes mellitus分类
医药卫生引用本文复制引用
Dai Cui,Wei Tang,Cuiping Liu,Kuanfeng Xu,Chao Liu..Effects of Glucagon-Like Peptide 1 on PDX-1, PAX-6 and NKx2.2 Gene Expressions in Isolated Pancreatic Islets[J].南京医科大学学报（英文版）,2006,20(3):141-144,4.基金项目
135 Project Foundation of Jiangsu Province (NO2001) （NO2001）
