首页|期刊导航|中国海洋湖沼学报(英文版)|Assessing the Germplasm of Laminaria (Phaeophyceae) with Random Amplified PoLymorphic DNA (RAPD) Method
中国海洋湖沼学报(英文版)2003，Vol.21Issue(2)：141-148,8.
Assessing the Germplasm of Laminaria (Phaeophyceae) with Random Amplified PoLymorphic DNA (RAPD) Method
Assessing the Germplasm of Laminaria (Phaeophyceae) with Random Amplified PoLymorphic DNA (RAPD) Method
摘要
Abstract
Eighteen gametophytes including L. japonica, L. ochotensis and L. longissima , were verified with random amplified polymorphic DNA (RAPD) technique. Eighteen ten base primers were chosen from 100 primers selected for final amplification test. Among the total of 205 bands amplified, 181 (88.3%) were polymorphic. The genetic distance among different strains ranged from 0.072 to 0.391. The dendrogram constructed by unweighted pair group method with arithmetic (UPGMA) method showed that the female and male gametophytes of the same cell lines could be grouped in pairs respectively. It indicated that RAPD analysis could be used not only to distinguish different strains of Laminaria , but also to distinguish male and female gametophyte within the same cell lines. There is ambiguous systematic relationship if judged merely by the present data. It seems that the use of RAPD marker is limited to elucidation of the phylogenetic relationship among the species of Laminaria.关键词
Laminaria/gametophyte/RAPD/germplasm identificationKey words
Laminaria/gametophyte/RAPD/germplasm identification分类
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赫英俊,邹俞萍,王晓东,郑治国,张大明,段德麟..Assessing the Germplasm of Laminaria (Phaeophyceae) with Random Amplified PoLymorphic DNA (RAPD) Method[J].中国海洋湖沼学报(英文版),2003,21(2):141-148,8.基金项目
Contribution No. 4389 from the Institute of Oceanology, Chinese Academy of Sciences.This research was supported by the State's High tech Project of China, Marine 863 project (No.819-03-02) and (2001AA621090). （No.819-03-02）
