中华医学杂志（英文版）2003，Vol.116Issue(9)：1413-1417,5.
Effects of estradiol on proliferation and metabolism of rabbit mandibular condylar cartilage cells in vitro
Effects of estradiol on proliferation and metabolism of rabbit mandibular condylar cartilage cells in vitro
程鹏 1马绪臣 1薛延 2李盛琳 3张祖燕1
作者信息
- 1. Center for Temporomandibular Disorders, Peking University School of Stomatology, Beijing 100081, China
- 2. Laboratory of Molecular Biology, Beijing Jishuitan Hospital, Beijing 100083, China
- 3. Laboratory of Oral and Maxillofacial Surgery, Peking University School of Stomatology, Beijing 100081, China
- 折叠
摘要
Abstract
Objective To investigate the effects in vitro of 17 β-estradiol (E2) on the proliferation and metabolism of rabbit mandibular condylar cartilage cells.Methods Chondrocytes were derived from neonatal rabbit mandibular condylar cartilage using a modified enzyme method. 17β-estradiol was added to the culture medium in a variety of concentrations. Cell growth and DNA, collagen, and proteoglycan synthesis were used as indicators of proliferation and differentiation of condylar chondrocytes. These were measured by cell number, 3H-proline and 35S-incorporation, respectively.Results E2 increased cell number and 3H-thymidine incorporation at 10-8 to 10-10 mol/L, and 10-8 to 10-11 mol/L in a dose-dependent manner, peaking at 10-8 mol/L and 10-9 mol/L, respectively. However, further increase in the concentration of estradiol caused inhibition of both cell number and 3H-thymidine incorporation, and this was significant at 10-6 mol/L. The effect of E2 on proteglycan synthesis was similar; the maximum stimulating effect was at 10-8 mol/L, and inhibition was significant at 10-6 mol/L. There was no obvious stimulatory effect of E2 on 3H-thymidine incorporation observed.Conclusions Estradiol affects condylar chondrocyte cell growth, DNA, and proteoglycan synthesis in a biphasic manner depending on its concentration. This indicates that estrogen may be important in the proliferation and differentiation of mandibular condylar chondrocytes, and could be relevant to some aspects of certain tempormandibular joint diseases by modulating the function of the chondrocytes.关键词
estradiol/cartilage/condyleKey words
estradiol/cartilage/condyle分类
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程鹏,马绪臣,薛延,李盛琳,张祖燕..Effects of estradiol on proliferation and metabolism of rabbit mandibular condylar cartilage cells in vitro[J].中华医学杂志（英文版）,2003,116(9):1413-1417,5.
