中山医科大学学报2001，Vol.22Issue(1)：14-18,5.
铅对肾上腺皮质细胞线粒体的氧化损伤
Mitochondria Oxidative Damage of Adrenocortical Cells
Induced b y Lead Acetate in vitro
杨杏芬 1庄志雄1
作者信息
- 1. 中山医科大学公共卫生学院卫生毒理学教研室,
- 折叠
摘要
Abstract
【Objective】 To study the effect of lead acetate on m itochondria oxidative stress and functional alteration of adrenocortical cells. 【Methods】 Male guinea pig fasciculata-glomerulosa (FG) cells were dispersed and primarily cultured as the testing system. The changes in the formation of re active oxygen species (ROS) and the mitochondria damage were observed when the c ells were incubated with lead acetate at different dosages of 0, 6.25, 12.5, 2 5, 50 and 100 μmol/L. The indices and their corresponding detecting methods inc luded: ① ROS (fluorescence spectrometry); ② Mitochondria membrane potential (M MP) and dead cells rate ［flow cytometry with dual-labeling of rhodamine 123(Rh 123) and propidium iodide(PI)］; ③ ATP level (chemoluminescence assay). 【Resul ts】 The formation of ROS in adrenocortical cells was increased in dose-depen dent manner after lead acetate incubation ［y=4.16+10.21×1g(x +1)，P<0.01，R2=0.641］；MMP decreased as the dose of PbAc increased . The mean fluorescent intensity(MFI) of Rh123 dropped from 1.01 to 0.91 as th e dosage increased from 6.25 μmol/L to 100 μmol/L, which were significantly lower than that of the control
(1.35, P<0.01)； Dead cells rate appeared to be slightly increased. Dead cells rates in the grou ps of 50 μmol/L and 100 μm ol/L PbAc were 3.16% and 3.40% respectively when compared with the control (1 .02%, P<0.05)；ATP levels tended to decrease, resulting from the synergist ic inhibition by the dose and incubation period of PbAc. The regression analysis showed that dose-effect relationship existed between the decreased ATP and the increased dosage of PbAc ［y=212965.7-51592.5×1g(x+1)，R 2=0.568，P <0.01］. 【Conclusion】 This study demonstrated that cytotoxicity of adrenoco rtical cells induced by lead acetate is probably attributed to mitochondria oxid ative stress. Mitochondria damage might be the early cellular and molecular even t in toxic effect of lead on adrenal cortex.关键词
铅/肾上腺皮质/氧化应激/线粒体/ATP/细胞毒性分类
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杨杏芬,庄志雄..铅对肾上腺皮质细胞线粒体的氧化损伤[J].中山医科大学学报,2001,22(1):14-18,5.
