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盐胁迫下8个转基因小黑杨株系的抗逆性比较

黄海娇 胡雪婷 李慧玉 姜静 李志新 王雷

森林工程2009,Vol.25Issue(4):14-18,5.
森林工程2009,Vol.25Issue(4):14-18,5.

盐胁迫下8个转基因小黑杨株系的抗逆性比较

Adverse Resistance Comparison of Eight Transgenic Populus simonii ×P. nigra Lines under NaCl Stress

黄海娇 1胡雪婷 1李慧玉 1姜静 1李志新 1王雷1

作者信息

  • 1. 林木遗传育种与生物技术教育部重点实验室,东北林业大学,哈尔滨,150040
  • 折叠

摘要

Abstract

Eight transgenic lines were selected from three kinds of transgenic Populus simonii ×P. nigra obtained before,including XC1, XB, XB2, XB3, XL1, XL5, XL6 and XL11, while non-transgenic ones were used as control. Treating them with 0.8% NaCl, the chlorophyll content and other main phsiological indicators were measured respectively on 6th day after stress. The results illustrated that the average chlorophyll content in transformants were 7.9% higher than that in the non-transformants. The activity of POD, CAT and APX was higher than the control while the damage degree of membrane in transformants was lower than the control. The activity of SOD in XL1, XL5, XL6 and XB4 were higher than the control. As a whole, the transformants had less injury degree of membrane and stronger anti-oxidative ability than the control.

关键词

小黑杨/转基因株系/MDA含量/POD活性/SOD活性

Key words

Populus simonii ×P. nigra/transgenic lines/MDA/POD/SOD

分类

农业科技

引用本文复制引用

黄海娇,胡雪婷,李慧玉,姜静,李志新,王雷..盐胁迫下8个转基因小黑杨株系的抗逆性比较[J].森林工程,2009,25(4):14-18,5.

基金项目

东北林业大学本科生创新性实验项目(2007038) (2007038)

黑龙江省科技计划项目(GB06B303 ()

WB07N02)资助 ()

森林工程

1001-005X

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