中国现代医学杂志2004，Vol.14Issue(18)：14-18,5.
胰岛素样生长因子-1对大鼠局灶性脑缺血再灌注后神经细胞凋亡及Bcl-2,Bax蛋白表达的影响
Effects of IGF-1 on apoptosis following cerebral ischemia and reperfusion in rats and its mechanism
张鸿 1郑东明 1赵冬雪 1丛琳 1王金春2
作者信息
- 1. 中国医科大学第二附属医院,神经内科,辽宁,沈阳,110004
- 2. 沈阳市第五人民医院,辽宁,沈阳,110004
- 折叠
摘要
Abstract
Objective: To study the effects of IGF-1 on apoptosis and the expression of Bcl-2 and Bax following cerebral ischemia and reperfusion and evaluate the protecting mechanism of IGF-1. Methods: The model of middle cerebral artery occlusion (MCAO) and reperfusion was set up. Thirty Wistar male rats were randomly divided into sham-operated group, ischemic group and IGF-1 treated group. IGF-1 of 10 μg was injected via caudal vein after 10 minutes with cerebral ischemia. The brain sections were used for TTC staining, TUNEL staining, Bcl-2 and Bax immunohistochemical staining. Results: Compared with ischemic group, the infarct volumes, the number of apoptotic cells and the expression of Bax positive cells in IGF-1 treated group were decreased significantly (P ＜0.01). The expression of Bcl-2 positive cells was increased significantly (P ＜0.01). Conclusions: IGF -1 can decrease apoptosis by up-regulating Bcl-2 and down-regulating Bax.关键词
胰岛素样生长因子-1/脑缺血再灌注/凋亡/Bcl-2/BaxKey words
insulin-like growth factor-1/cerebral ischemia and reperfusion/apoptosis/Bcl-2/Bax分类
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张鸿,郑东明,赵冬雪,丛琳,王金春..胰岛素样生长因子-1对大鼠局灶性脑缺血再灌注后神经细胞凋亡及Bcl-2,Bax蛋白表达的影响[J].中国现代医学杂志,2004,14(18):14-18,5.
