中国临床康复2005，Vol.9Issue(36)：142-144,3.
低氧复合常氧训练增强大鼠腓肠肌琥珀酸脱氢酶的活性
Enhanced activity of succinate dehydrogenase in rat's gastrocnemius under hypoxic living with normoxic training
摘要
Abstract
BACKGROUND: The effect of hypoxic training on energy metabolism of skeletal muscles is receiving much interest from researchers of sports medicine. But at present, sufficient evidence supporting hypoxic training for its improvement on skeletal muscle aerobic oxidation and anaerobic capacity has not been obtained.OBJECTIVE: To investigate the effect of hypoxic living with normoxic training on aerobic oxidation and glycolytic capacity of rat skeletal muscles.DESIGN: Randomized controlled experiment.SETTING: College of Human Sport Science of Beijing Sport University.MATERIALS: This experiment was carried out in the Hebei Institute of Sport Science between March and July, 2003. Totally 96 normal male SD rats of 7 weeks were randomized into control group (C), hypoxic control group (HC), hypoxic living with normoxic training group (HNT) and normoxic training group (NT). The latter 3 groups were further divided into 3 subgroups (n=8) according to the sampling time points of 4, 7, and 28 days.METHODS: Hypoxic living with normoxic training protocol was designed to simulate the moderate-intensity normoxic training with hypoxic living for 1 hour in the condition of 4000 m altitude (with the slope of the treadmill of 10%, speed of 20 m/minutes performed once daily, 5 times a week for 4 consecutive weeks). Gastrocnemius muscle samples (0.5 g) were collected at the conjunction of the red and white tissues from the rats at the specified time points. Ultraviolet spectrophotometry was used to determine the activity of succinate dehydrogenase (SDH) in 10% gastrocnemius muscular homogenate. Gastrocnemius lactate dehydrogenase (LDH) activity was assayed by the "rate method" in the mode setting of the semi-automatic biochemical analyzer. The total protein density was determined with "endpoint method" with the analyzer, namely biuret method.MAIN OUTCOME MEASURES: Changes of the activity of SDH and LDH in rat gastrocnemius muscles at different time points.RESULTS: None of the animals died during the experiment and all entered the result analysis. Hypoxia .alone could increase SDH activity,which, however, tended to decrease with time. In contrast, hypoxic with normoxic training obviously increased SDH activity during the entire period of 4-28 days, and the changes in SDH, along with those in hypoxia group, depicted two curves crossing each other in the fashion of a scissor.At day 28, SDH activity was approximately twice as high as that of the control group. Both hypoxic alone and normoxic training alone could reduce LDH activity, with remained stable at around the normal level in rats with hypoxic living with normoxic training normoxic training.CONCLUSION: Hypoxic living with normoxic training can promote the aerobic metabolic potential but has no significant effect on LDH activity in rats under the same training condition and exercise load, in other words, the glycolytic capacity of the skeletal muscle can not be necessarily improved after 4-week hypoxic living with normoxic training normoxic training.关键词
大鼠/骨骼肌/琥珀酸脱氢酶/乳酸脱氢酶分类
医药卫生引用本文复制引用
毛杉杉,潘同斌,王瑞元..低氧复合常氧训练增强大鼠腓肠肌琥珀酸脱氢酶的活性[J].中国临床康复,2005,9(36):142-144,3.基金项目
国家体育总局应用基础研究项目(04032) the Grant from the Program of Applied Basic Study of State Sports General Administration, No.04032 （04032）
