作物学报2006，Vol.32Issue(10)：1430-1436,7.
渗透胁迫对玉米幼苗叶片不同形态多胺含量的影响
Effect of Osmotic Stress on the Contents of Different Form Polyamines in Leaves of Maize Seedlings
摘要
Abstract
The changes in contents and forms of polyamines (PAs) in seedling leaves of two maize (Zea mays L.) cultivars, Nongda 108 (drought-tolerant) and Yedan 13 (drought-sensitive), were investigated under osmotic stress. On the 8th day after polyethylene glycol (PEG) -6 000 treatment, free spermidine (fSpd) and free spermine (fSpm) content in leaves increased significantly(P＜0.05)in Nongda 108, while Yedan 13 showed a significant increase of free putrescine (fPut) content. Thus Nongda 108 gave a higher ratio of (fSpd + fSpm)/fPut than Yedan 13 in leaves under osmotic stress. Exogenous methylglyoxal-bis (guanylhydrazone) (MGBG), which is an S-adenosylmethionine decarboxylase (SAMDC) inhibitor, resulted in reduction of fSpd and fSpm contents and aggravation of PEG-induced injury to Nongda 108 leaves, while exogenous Spd treatment resulted in an increase of fSpd + fSpm in leaves, in concert with an alleviation of PEG-induced injury to seedlings of Yedan 13. Osmotic stress induced significant increases of acid soluble conjugated PA (AS-conjugated PA) and acid insoluble bound PA (AIS-bound PA) contents in leaves of Nongda 108, and osmotic stress affected slightly in Yedan 13 leaves. Exogenous phenanthrolin (o-Phen), an inhibitor of transglutaminase (TGase), aggravated PEG-induced injury to Nongda 108, coupled with decreases of AIS-bound PA content. The results suggest that fSpd, fSpm, AS-conjugated-PA and AIS-bound PA facilitate the osmotic stress tolerance of maize seedlings.关键词
玉米/多胺形态/渗透胁迫Key words
Maize/Polyamine forms/Osmotic stress分类
农业科技引用本文复制引用
刘怀攀,纪秀娥,刘天学,史留功,李潮海..渗透胁迫对玉米幼苗叶片不同形态多胺含量的影响[J].作物学报,2006,32(10):1430-1436,7.基金项目
Special Important Program of Ministry of Science and Technology (2004BA502A) and China Postdoctoral Science Foundation (2005037633). （2004BA502A）
