华东理工大学学报（自然科学版）2001，Vol.27Issue(2)：143-146,4.
酶法合成抗癌新药氟铁龙前体——5-FUR
Enzymatic Synthesis of 5-FUR, a Precursor of a New Anti-cancer Medicine 5-DFUR
闫蓬勃 1丁庆豹 1邱蔚然 1严滢 1石佳瑛1
作者信息
摘要
Abstract
A strain of Brevibacterium acetylium QDY2000 that can produce nucleoside phosphorylase has been successfully screened. 5-Fluorouridine which can be used as the precursor of the 5′-deoxy-5-fluorouridine was enzymatically synthesized from guanosine and 5-fluorouracil by QDY2000. The optimal reaction conditions were studied in 150mL shaking bottle with 15mL solution. The conversion yield could reach above 60% while the reaction mixtures, containing 20～30g/L wet cells, 100mmol/L substrates and 50mmol/L phosphate buffer (pH adjusted to 8.0 with NaOH), were incubated at 60°C for 4h at the shaking speed of 160r/min.
关键词
酶法合成/5-氟尿苷/乙酰短杆菌/5-氟尿嘧啶/鸟苷分类
生物科学引用本文复制引用
闫蓬勃,丁庆豹,邱蔚然,严滢,石佳瑛..酶法合成抗癌新药氟铁龙前体——5-FUR[J].华东理工大学学报（自然科学版）,2001,27(2):143-146,4.
