中华医学杂志（英文版）2007，Vol.120Issue(16)：1444-1447,4.
Growth activity of epidermal cells from different parts of human body
Growth activity of epidermal cells from different parts of human body
摘要
Abstract
Background Most epidermal cells used in skin tissue engineering are obtained from the skins of fetuses or prepuces,which can not be widely used in culturing and transplanting autologous epidermis for patients with extensive burn wounds. To solve the problem, in this study, we cultured epidermal cells from different parts of human body in vitro, and detected their growth activity.Methods Normal epidermal cells obtained from the prepuce, scalp, and axilla of male patients, were cultured and passaged. Their growth characteristics including adherent rate and growth activity were compared. Data were analyzed by homogeneity test of variance.Results In primary culture, the growth of epidermal cells from the prepuce was significantly faster than that of the epidermal cells from the scalp and axilla. In the cells obtained from the prepuce, 80% confluence was achieved on day 12, while on day 16 and day 20 in the cells from the scalp and axilla, respectively. However, no significant difference was detected in their growth and proliferation in the second passage.Conclusions Although the growth of epidermal cells obtained from the scalp and axilla is slower than that from the prepuce in primary culture, stable cell line can be established and used in preparation of auto-epidermal grafts for patients with extensive burn wounds. Therefore, the scalp and axillary skin should be considered as important sources of epidermal cells other than the prepuce.关键词
epidermis/ cell growthKey words
epidermis/ cell growth分类
医药卫生引用本文复制引用
CHAI Jia-ke,SHENG Zhi-yong,MA Zhong-feng,YANG Hong-ming,LIU Qiang,Liang Li-ming..Growth activity of epidermal cells from different parts of human body[J].中华医学杂志（英文版）,2007,120(16):1444-1447,4.基金项目
This work was supported by the grants from the Fund for Major Subject in Clinical Science and Technology of PLA (No.2002-35),Funds for Advancement of Medical Science of the Capital (No.2002-2036, 2005-2040), Beijing Fund for Advance in Science and Technology (No. H060920050830) and the Specific Fund for Military 11.5 Project of Medical Science and Technology (No.06Z054) （No.2002-35）
