中华医学杂志（英文版）2003，Vol.116Issue(1)：145-147,3.
The relationship between MTHFR gene polymorphisms, plasma homocysteine levels and diabetic retinopathy in type 2 diabetes mellitus
The relationship between MTHFR gene polymorphisms, plasma homocysteine levels and diabetic retinopathy in type 2 diabetes mellitus
孙家忠 1徐焱成 1朱宜莲 1鲁红云 1邓浩华 1范幼筠 1孙苏欣 1张颖1
作者信息
- 1. Department of Endocrinology, Zhongnan Hospital, Wuhan University, Wuhan 430071, China
- 折叠
摘要
Abstract
Objective To evaluate the role of methylenetetrahydrofolate reductase (MTHFR) gene polymorphisms and plasma homocysteine levels in patients with type 2 diabetes mellitus and diabetic retinopathy (DR). Methods Total of 208 patients with type 2 diabetes mellitus and 57 controls were recruited into the study. MTHFR genetic C677T polymorphisms were determined by PCR-RFLP. Plasma total homocysteine levels were measured using high-performance liquid chromatography (HPLC) with fluorescence detection. Results The frequencies of MTHFR TT homogeneous type, CT heterogeneous type and allele T (28.18%, 41.82%, 49.09%) were significantly higher in the type 2 diabetes mellitus with diabetic retinopathy group than those without retinopathy (18.37%, 29.59%, 33.16%) and those of controls (17.54%, 28.07%, 31.58%). The presence of the T allele appeared to have a strong association with the development of diabetic retinopathy. The odds ratio was 1.94 with a 95% confidence interval of 1.31-2.88. Moreover, plasma homocysteine levels were remarkably higher in patients with TT or CT genotype than in patients with the CC genotype. Conclusion MTHFR gene C677T mutation associated with a predisposition to increased plasma homocysteine levels may be considered as a genetic risk factor for diabetic microangiopathy (such as DR) in Chinese patients with type 2 diabetes mellitus.关键词
diabetic retinopathy/methylenetetrahydrofolate reductase gene/homocysteineKey words
diabetic retinopathy/methylenetetrahydrofolate reductase gene/homocysteine分类
医药卫生引用本文复制引用
孙家忠,徐焱成,朱宜莲,鲁红云,邓浩华,范幼筠,孙苏欣,张颖..The relationship between MTHFR gene polymorphisms, plasma homocysteine levels and diabetic retinopathy in type 2 diabetes mellitus[J].中华医学杂志（英文版）,2003,116(1):145-147,3.
