中德临床肿瘤学杂志(英文版)2002，Vol.1Issue(3)：145-148,4.
DLI治疗CML病人后的克隆性增殖TCRVβ细胞分析
Analysis of the Clonal Expansion of TCR Vβ T Cells in Patients with CML after DLI
摘要
Abstract
Objective To investigate the clonal expansion of T cell receptor (TCR) Vβ subfamily T cells which were considered as GVL effective cells after donor lymphocytes infusion (DLI) in patients with relapse chronic myelogenous leukemia (CML) after allogeneic bone marrow transplantation (allo-BMT). Methods The CDR3 of TCR Vβ24 subfamily genes were amplified in samples of peripheral blood mononuclear cells at different time points before and after DLI, which were drawn from 2 cases of relapse CML treated by allo-BMT, to observe the usage of TCR Vβ repertoire. The PCR products were further labeled with fluorescent and analyzed by genescan technique for identification of the CDR3 size, to evaluate the clonality of the detectable TCR Vβ T cells. Results Only 4-11 Vβ subfamily T cells could be identified in CML cases before DLI, and 12-21 Vβ subfamily T cells could be detected in samples from CML which display remission after DLI. Genescan analysis showed that new clonal expansion TCR Vβ subfamily T cells could be found in samples after DLI. Conclusion The skew distribution of TCR Vβ subfamily T cells could be found on patients with relapse CML after allo-BMT, and this skewing pattern may stage to stage to normal pattern during the complete remission. The GVL effect may exert through some clonal expansion TCR Vβ subfamily T cells during the treatment of DLI in relapse CML.关键词
供者淋巴细胞输注/慢性粒细胞白血病/T细胞受体β基因/T细胞克隆/异基因骨髓移植Key words
donor lymphocytes infusion/chronic myelogenous leukemia/T cell receptor Vβ gene/T cell clonality/allogeneic bone marrow transplantation分类
医药卫生引用本文复制引用
李扬秋,杨力建,陈少华,张涛,许敏华,罗更新..DLI治疗CML病人后的克隆性增殖TCRVβ细胞分析[J].中德临床肿瘤学杂志(英文版),2002,1(3):145-148,4.基金项目
This work was supported by the grants from National Education Ministry (No. 200065) and Department of Education of Guangdong Province (No. 200021), and from the Key Project Foundation of the Science and Technology Commission of Gaungdong Province (No. 2KM （No. 200065）
