首页|期刊导航|中国农业科学(英文版)|Effects of Exogenous Jasmonic Acid on Concentrations of Direct-Defense Chemicals and Expression of Related Genes in Bt(Bacillus thuringiensis)Corn(Zea mays)
中国农业科学(英文版)2007，Vol.6Issue(12)：1456-1462,7.
Effects of Exogenous Jasmonic Acid on Concentrations of Direct-Defense Chemicals and Expression of Related Genes in Bt(Bacillus thuringiensis)Corn(Zea mays)
Effects of Exogenous Jasmonic Acid on Concentrations of Direct-Defense Chemicals and Expression of Related Genes in Bt(Bacillus thuringiensis)Corn(Zea mays)
摘要
Abstract
Bt corn is one of the top three large-scale commercialized transgenic crops around the world.It is increasingly clear that the complementary durable approaches for pest control,which combine the endogenous defense of the crop with the introduced foreign genes,are promising alternative strategies for pest resistance management and the next generation of insect-resistant transgenic crops.In the present study,we tested the inducible effects of exogenous jasmonic acid(JA) on direct-defense chemical content,Bt protein concentration,and related gene expression in the leaves of Bt corn cultivar 34B24 and non-Bt cultivar 34B23 by chemical analysis,ELISA,and RT-PCR.The results show that the expression of LOX,PR-2αMPI,and PR-1 genes in the treated leaf(the first leaf)was promoted by exogenous JA both in 34B24 and 34B23.As compared with the control,the concentration of DIMBOA in the treated leaf was significantly increased by 63 and 18% for 34B24 and 34B23,respectively.The total phenolic acid was also increased by 24 and 12% for both 34B24 and 34B23.The Bt protein content of 34B24 in the treated leaf was increased by 13% but decreased significantly by 27% in the second leaf.The induced response of 34B24 was in a systemic way and was much stronger than that of 34B23.Those findings indicated that there is a synergistic interaction between Bt gene and internally induced chemical defense system triggered by externally applied JA in Bt corn.关键词
Bt corn/jasmonic acid(JA)/defense chemicals/defense genesKey words
Bt corn/jasmonic acid(JA)/defense chemicals/defense genes分类
农业科技引用本文复制引用
FENG Yuan-jiao,WANG Jian-wu,LUO Shi-ming..Effects of Exogenous Jasmonic Acid on Concentrations of Direct-Defense Chemicals and Expression of Related Genes in Bt(Bacillus thuringiensis)Corn(Zea mays)[J].中国农业科学(英文版),2007,6(12):1456-1462,7.基金项目
This work was supported by the National Natural Science Foundation of China(30470335,30770402) （30470335,30770402）
and Natural Science Foundation of Guangdong Province,China(E039254,06025813). （E039254,06025813）
