中国临床康复2005，Vol.9Issue(36)：147-149,3.
经皮穴位电刺激后鼠耐力训练中体能的变化
Changes in physical fitness of mice and rats in endurance training after transcutaneous electrical stimulation
摘要
Abstract
BACKGROUND: Sport fatigue has been all along the hotspot and difficulty in the study of sport medicine. To eliminate sport fatigue and prevent from excessive fatigue effectively and in time is related directly to the results of sport training, improvement of sport achievement and health of athletes. It has been verified during long-term medical practice that the therapies in Chinese medicine are safe, effective, convenient and economical, in which, acupuncture is limited in the treatment of sport injury and sport disease in sport medicine. But, there is few study of it on prevention and treatment of fatigue.OBJECTIVE: Due to the limitation of acupuncture in practical training,this experiment is to observe the effects of atraumatic transcutaneous acupoint electrical stimulation on physical fitness in mice and rats.DESIGN: Randomized controlled experiment was designed.SETTING: Department of Sports Medicine, Wuhan Institute of Physical Education;College of Acupuncture & Massage, Shanghai University of Traditional Chinese MedicineMATERIALS: The experiment was performed in Acupuncture and Massage College of Shanghai University of Traditional Chinese Medicine from June 2002mice of 4 weeks old, which was randomized into blank control and transcutaneous acupoint electrical stimulation group (TAES group), 10 mice in each one.blank control, training control and TAES group, 10 rats in each one.METHODS: Effect on loaded swimming time and hypoxia endurance time:G6805-A electric acupuncture apparatus (bipolar non-parallel pulsating, 0.5ms in width, 2V of peak value in pulsating voltage, randomized alternated disperse-dense wave at the range of 2 to 30 Hz) was used. The points selected (corresponding to baihui and mingmen in human body) were stimulated for 15 minutes before being trained every day in TAES group on Kunming mice.The mouse was placed in a swimming trough, 30 cm deep, at (25±0.5)℃ of water temperature, with 10% load of body mass on the tail for loaded swimming. Exhausted swimming time was recorded with stopwatch from the beginning of experiment to the exhaustion. The identifying criteria of exhausted swimming time: when the small white mouse was exhausted, the time was recorded since its head was unable to expose on the water surface till it was sunk to the bottom. The training lasted 7days. After the last experiment, the mouse was placed in a 50 mL airtight glass bottle with 10 g lime sodium and the time of breathing stop was recorded with second (asphyxia time). Effect on desk fatigue time and body mass in rats: in TAES group and training control, the rats exercised on the animal running desk at 15-27 m/min, 30 min/time, for 14 days continuously. The exercise stopped once on the 7th day and the 13th day respectively. The transcutaneous electric stimulus was same as the above. Identifying criteria of fatigue: the rat could not followed prearranged speed after the stimulation and retarded on the posterior half section on the desk, and it was stimulated with electricity over 3 times. Body mass of rat was measured with common scale before and after experiment.MAIN OUTCOME MEASURES: Exhausted swimming time and hypoxia endurance time of mice before and after experiment, desk fatigue time and change in body mass in rats.RESULTS: All of the animals accomplished the experiment smoothly and and hypoxia endurance time: the exhausted time in TAES group on the 1st and 7th days was prolonged significantly compared with blank control group [(38.78±4.33), (30.08±3.12) minutes; P ＜ 0.01]; [(41.35±3.45), (30.11 ±3.23)minutes, P ＜ 0.01]. The exhausted time in TAES group on the 7th day was longer remarkably than that on the 1st day (P ＜ 0.05). The hypoxia endurance time in TAES group was longer significantly than blank control fatigue time and body mass in rats: the difference of fatigue time before and after experiment was not big in blank control group (P ＞ 0.05) and the difference was remarkable before and after experiment in training control and TAES groups (P ＜ 0.05). Antifatigue time after experiment in TAES group and training control group was prolonged remarkably compared with blank control group [(165.50±13.70),(101.50±21.87), (23.33±10.46)minutes, P ＜ 0.01]. The body mass of rat was increased at different degrees after experiment compared with that before experiment in each group (P ＜0.05), of which, the increasing amplitude of rat mass in TAES was less than the rest two group, but without significant difference.CONCLUSION: A significant improvement is achieved in TAES group no matter in swimming exhausted time, desk fatigue time, or in hypoxia endurance time. The body mass is increased remarkably after experiment, but the increasing amplitude in TAES group is the smallest, without significant difference. Transcutaneous acupoint electrical stimulation is a kind of rational integration of acupuncture in Chinese medicine with physical therapy,characterized as safety, convenience, practice and efficacy.关键词
穴位/皮电刺激/耐力训练分类
医药卫生引用本文复制引用
杨翼,李章华,何金森..经皮穴位电刺激后鼠耐力训练中体能的变化[J].中国临床康复,2005,9(36):147-149,3.基金项目
湖北省科学技术研究计划项目(D200533002) Science and Technology Research Program of Hubei Province, No. D200533002 （D200533002）
