北京大学学报（医学版）2001，Vol.33Issue(2)：147-149,3.
尾加压素Ⅱ对大鼠主动脉一氧化氮合酶/一氧化氮系统的影响
Effect of urotensin Ⅱ on nitric oxide synthese/nitric oxide pathway in rat aorta
姜志胜 1张勇刚 1齐永芬 1许松 2徐少平 3庞永政 1唐朝枢1
作者信息
- 1. 北京大学第一医院心血管病研究所，北京 100034
- 2. 北京大学第一医院中心实验室，北京 100034
- 3. 中国人民解放军北京军区总医院心内科
- 折叠
摘要
Abstract
Objective: To study the effect of urotensin(U Ⅱ) on nitric oxide synthase(NOS)/nitric oxide(NO) system in rat aorta. Methods: The aortic slices were incubated in vitro. The medium nitrite content, vascular NOS activities and cGMP content were measured. Results: After incubation of aorta for 1.5－3.0 h, UⅡ(10－9－10－7 mol*L－1) increased nitrite production ( 14.8%－80.9%, P<0.01) in dose-dependent manner. U Ⅱ significantly stimulated the total NOS activities, mainly activated constitutive NOS (P<0.05 or P<0.01), however, the inducible NOS activities were not altered (P>0.05). Incubation of aortic slices with U Ⅱ (10－9－10－7 mol*L－1) increased vascular cGMP content in dose-dependent manner. Co-incubation vascular tissue with U Ⅱ and GN-L-nitro-arginine, an inhibitor of NOS, obviously inhibited the U Ⅱ-induced NOS activation, NO production and cGMP formation. Conclusion: The results suggest that U Ⅱ can activate the vascular NOS/NO pathway, increasing tissue NO production and cGMP content.关键词
尾加压素Ⅱ/一氧化氮合酶/一氧化氮/代谢/血管升压素/药理学/主动脉/化学分类
医药卫生引用本文复制引用
姜志胜,张勇刚,齐永芬,许松,徐少平,庞永政,唐朝枢..尾加压素Ⅱ对大鼠主动脉一氧化氮合酶/一氧化氮系统的影响[J].北京大学学报（医学版）,2001,33(2):147-149,3.
