中国药理学通报2001，Vol.17Issue(2)：147-150,4.
氯通道阻断剂对α1-肾上腺素受体亚型引起的Ca2 +内流的影响
Effects of Cl--channels blockers on Ca2+ influx induced by α1-adre noceptor subtypes
阮红梅 1关永源 2贺华 2丘钦英2
作者信息
- 1. 广州医学院生理学教研室,
- 2. 中山医科大学药理学教研室,
- 折叠
摘要
Abstract
AIM To investigate the roles of Cl- channels in C a2+ influx induced by activaion of α1-adrenoceptor subtypes in transfe cted-CHO cells.METHODS The effects of drugs on α1A、α1 B and α1D-AR-induced Ca2+influx were investigated with Fura-2 fluorescence technique. RESULTS The α1A-AR-induced Ca2 + influx was inhibited by furosemide（2.5～10 μmol·L-1）and SK&F96365 （5～15 μmol·L-1）in a concentration-dependent manner respectively；The α1B-AR-induced Ca2+ influx could also be inhibited by NFA（2.5 ～10 μmol·L-1），whereas the α1D-AR-induced Ca2+ influx w as only suppressed by NFA.In α1B-CHO cells，Adr-triggered Ca2+ in flux could be further inhibited by NFA or furosemide after the maximal inhibitio n by SK&F96365；SK&F96365 could further inhibit Ca2+ influx which had been inhibited by NFA or furosemide. In α1A-CHO cells，Adr-triggered Ca 2+ influx could be further inhibited by SK&F96365 after had been inhibited by furosemide； furosemide could not further inhibite Ca2+ influx which had b een inhibited by S&F96365. CONCLUSION There are different character istics of Cl- channels related to α1A、 α1B and α1D-AR- induced Ca2+ influx.关键词
钙/Cl-通道/α1-肾上腺素受体分类
医药卫生引用本文复制引用
阮红梅,关永源,贺华,丘钦英..氯通道阻断剂对α1-肾上腺素受体亚型引起的Ca2 +内流的影响[J].中国药理学通报,2001,17(2):147-150,4.
