中华医学杂志（英文版）2002，Vol.115Issue(10)：1475-1478,4.
骨髓增生异常综合征的骨髓细胞端粒酶活性研究
Telomerase activity in myelodysplastic syndrome
摘要
Abstract
Objective To study telomerase activity (TA) and its variation in bone marrow mononuclear cells from patients with myelodysplastic syndrome (MDS) at different stages in comparison with normal bone marrow cells and leukemic cells. Methods The TA was semi-quantitatively determined in mononuclear cells from 20 normal bone marrow samples, 21 patients with MDS at different stages and 32 cases of acute leukemia by using a polymerase chain reaction-enzyme linked immuno-sorben assay (PCR-ELISA) kit. Results The TA in normal bone marrow cells was in the range of 0 to 0.3 units (U) with a mean of 0.11±0.08 U. Among them, 3 samples were considered positive in accordance with the standard recommended by the kit's pamphlet. In bone marrow cells from patients with acute leukemia, the TA was ranging from 0 to 0.96 U with a mean value of 0.42±0.26 U. The positive rate was 78.1% which was signific antly different from that in normal bone marrow (BM) (P<0.01). In case of myelodysplastic syndrome, the average level of TA was 0.27±0.19 U (ranging fr om 0 to 0.97 U) with a positive rate of 66.7%. In comparison with normal BM cells, the difference was significant (P<0.05). Particularly, the MDS high -risk subgroup exhibited a significantly higher activity of telomerase (P<0 .05). In comparison with INT-1 and INT-2 subgroups in MDS patients based on international prognostic scoring system (IPPS), the difference in TA was also si gnificant (P<0.05). The abnormality in cell karyotype was not correlated w ith TA.Conclusion The normal bone marrow cells demonstrate TA at a marginal level while a remarkab ly increasing level may be seen in acute leukemia patients. The BM cells from M DS patients display a moderate TA among which the high risk MDS subgroup with a poor prognostic IPPS score exhibited markedly higher TA.关键词
端粒酶/骨髓增生异常综合征/急性白血病Key words
telomerase activity/myelodysplastic syndrome/acute leukemia分类
医药卫生引用本文复制引用
傅琤琤,陈子兴..骨髓增生异常综合征的骨髓细胞端粒酶活性研究[J].中华医学杂志（英文版）,2002,115(10):1475-1478,4.基金项目
This study has been supported by the grant E9705 from the Suzhou Medical College and the grant of "333 Human Resource Engineering Foundation" from the Jiangsu Province Government （）
