作物学报2008，Vol.34Issue(8)：1480-1483,4.
ISSR在高梁属植物遗传关系研究中的应用
Application of ISSR in Genetic Relationship Analysis of Sorghum Species
摘要
Abstract
The genetic relationship among six sorghum species, namely, S. bicolor, S. bicolor×S, sudanese, S. saccharatum, S.sudanese, S. almum, and S. halepense was analyzed by inter-simple sequence repeat (ISSR) method. The results showed that the diversity of sorghum was high at DNA level. Twenty-two primers selected from 110 ISSR primers could amplify 182 clear and reproducible bands, of which 153 bands were polymorphic, accounting for 84.0%. All the sorghum species studied could be distinctly divided into two major groups with the genetic distance level at 0.25 by cluster analysis based on the Neighbor-Joining method. Some primers produced highly polymorphic band patterns in different species, such as IR 89, ISI6, according to them, all the sorghum species used could be identified. The results could be used in classification, identification and evolution of sorghum.关键词
高粱属植物/ISSR/遗传关系Key words
Sorghum/ISSR/Genetic relationship分类
农业科技引用本文复制引用
方雪恩,陈沁,印丽萍,王伟..ISSR在高梁属植物遗传关系研究中的应用[J].作物学报,2008,34(8):1480-1483,4.基金项目
Supported by Shanghai Science and Technology Committee (04DZ05003) （04DZ05003）
