基础医学与临床2001，Vol.21Issue(2)：148-150,3.
褪黑激素对17-β-雌二醇诱致的大鼠垂体催乳素瘤细胞增殖的抑制作用
Inhibitory effects of Melatonin on the proliferation of 17-β-estrogen-induced pituitary prolactin-secreting tumor cells in rats
摘要
Abstract
In present study,we have examined the effect of melatonin at different concentrations on the proliferation of 17-β-estrogen(E2)-induced prolactinoma cells of rats.The prolactinomas were established by implanting E2-laden silastic capsule subcutaneously in 80～100 g weight Sprague-Dawley (SD) male rats.Ninety days after E2 capsules implantation,the animals were killed,and prolactinomas were removed.The suspension of tumor cells was obtained by enzymatic digestion,filtration,centrifugation,and resuspension in incubating medium.1 ml aliquot of the cell suspension containing 0.75×106 cells were distributed into each well of 24 well cell culture plate respectively.Then MLT was added to appropriate wells to make the final concentration 10-5 to 10-13 mol/L.Respective quantity of ethanol was added to control wells.Then the cells were incubated at 37℃ in 5 % CO2.After 1 h,74KBq(2μCi) / 2 μL of ［3H］-TdR was added in 50 μL of the medium.The incubation was terminated 24 h later,and then the radioactivity was counted.The result showed that the radioactivity (counts/min) of ［3H］-TdR in tumor cells in control group and test group at doses of 10-5 ,10-7 ,10-9 ,10-11 and 10-13 mol/L melatonin were 2038.75 ±186.84,1421.75 ±64.49,1238.25 ± 61.72,924 ± 64.44,1033.25± 127.22 and 1103.25 ±151.53,respectively.The results suggested that Melatonin in the concentration range 10-5～10-13 mol/L could inhibit the proliferation of 17-β-estrogen-induced rat prolactinoma cells.关键词
褪黑激素/催乳素瘤/细胞增殖/［３H］-TdR掺入分类
生物科学引用本文复制引用
高列,单惠敏,许荣焜,许建萍..褪黑激素对17-β-雌二醇诱致的大鼠垂体催乳素瘤细胞增殖的抑制作用[J].基础医学与临床,2001,21(2):148-150,3.基金项目
国家自然科学基金(39770287；39870341)；卫生部科学基金(96-1-007) （39770287；39870341）
