中国免疫学杂志2001，Vol.17Issue(3)：148-151,4.
表达幽门螺杆菌hpaA重组减毒鼠伤寒沙门氏菌的构建和鉴定
Construction and identification of a recombinant attenuated Salmonella typhimurium vaccine strain expressing Helicobacter pylori hpaA gene
摘要
Abstract
Objective: To construct a recombinant live attenuated Salmonellaty phimurium vaccine strain expressing Helicobacter pylori hpaA gene.Methods: By g enetic engineering methods,hpaA gene was cloned into a procaryotic expression pl asmid pTrc99A,and the identified recombinant plasmid was then used to transform an attenuated Salmonella typhimurium vaccine strain SL3261,and the positive clon es were screened by PCR and restriction enzyme digestion. HpaA expression was an alyzed by SDS-PAGE and Western blot.Results:Confirmed by PCR and restriction en zyme digestion ,a recombinant procaryotic expression plasmid pTrc99A-hpaA was c o nstructed,and then introduced into an attenuated Sa lmonella typhimurium vaccine strain SL3261 successfully. HpaA was expressed in t he recombinant strains in the form of a dimer,and also its immunogenicity was co nfirmed by Western blot. Conclusion: A recombinant live attenuated Salmonella ty phimurium vaccine strain expressing Helicobacter pylori hpaA gene was constructe d and identified,and this work will help to develop an oral recombinant live vac cine against Helicobacter pylori.关键词
幽门螺杆菌 hpaA基因 鼠伤寒沙门氏菌 疫苗 重组分类
医药卫生引用本文复制引用
朱森林,陈旻湖,廖文俊,陈洁,胡品津..表达幽门螺杆菌hpaA重组减毒鼠伤寒沙门氏菌的构建和鉴定[J].中国免疫学杂志,2001,17(3):148-151,4.基金项目
本研究为卫生部临床学科重点项目(No.97040226)、广东省科技计划重点 攻关项目(No.99No.4802G)和广东省自然科学基金课题(No.990077) （No.97040226）
