中国全科医学2009，Vol.12Issue(16)：1486-1488,3.
小鼠组织因子原核质粒的构建和重组蛋白的表达
Prokaryotic Vector Construction and Expression of Murine Tissue Factor
陈少兴 1谭光宏 2黎岳南 1黄风迎 1王华 3黄用豪1
作者信息
- 1. 571101,海南省海口市,海南医学院海南省热带病重点实验室
- 2. 海南医学院附属医院内科
- 3. 海南医学院基础部病理教研室
- 折叠
摘要
Abstract
Objective To construct a prokaryotic vector encoding murine tissue factor and to express the recombinant tissue factor protein.Methods The cDNAs of murine tissue factor were amplified from total RNAs of murine livers.The corresponding prokaryotic expression vectors were constructed by restrictive endonuclease digestion and ligation,and then the recombinant vectors pQE-mTF were proofed by restricting enzymatic digestion and DNA sequencing.The confirmed vectors were transfected into E.coli JM109 and recombinant protein expression was induced by Isopropyl-β-D-thiogalactoside (IPTG).Results The recombinant expression vectors of murein tissue factor(pQE-mTF)were constructed successfully.The recombinant murine tissue factor protein was expressed stably in E.coli JM109.Conclusion Recombinant murine tissue factor lays a solid foundation for applying tissue factors to treatment and further functional study.关键词
组织因子/重组蛋白质/原核表达载体/小鼠Key words
Tissue factor/Recombinant protein/Prokaryotic expression vector/Mice分类
医药卫生引用本文复制引用
陈少兴,谭光宏,黎岳南,黄风迎,王华,黄用豪..小鼠组织因子原核质粒的构建和重组蛋白的表达[J].中国全科医学,2009,12(16):1486-1488,3.
