生命科学研究2001，Vol.5Issue(2)：149-154,6.
重组人促红细胞生成素纯化工艺的优化
Optimized Purification Process of Human Recombinant Erythropoietin
李干祥 1聂东宋 2杨葆生 1朱宁1
作者信息
- 1. 深圳市斯贝克生物药业有限公司,
- 2. 湖南师范大学生命科学学院,
- 折叠
摘要
Abstract
Gene-engineered CHO cell line ZK9703 with the capacity to express huma n erythropoietin were cultured in packed bed bioreactor in a perfusion culture m ode. Harvest medium was collected and purified with a three-step purificati on pr ocession-exchanger, reverse phase and molecular sieve chromatogra phy. Three lot s of harvest medium was purified with a purification processQ-Sep harose XL, C4 reverse phase and S-200( the first method), three lots of harvest medium wa s pu rified with a purification processDEAE FF, source reverse phase an d S-200(the second method),the final products were analyzed for its purity and specific in vitro biological activity. Its purity is over 98%, and its specific in vit ro bio logical activity is over 1.3×105 IU/mg.The total recovery of EPO activity wa s 23.56% (by the fist method) and 28.57%.( by the second method), respectively. Th e results show that the second method of purification process is rapid and effic ient with high recovery, and could be more useful in large-scale production of rhEPO.关键词
重组人促红细胞生成素(rhEPO)/纯化工艺/回收率分类
生物科学引用本文复制引用
李干祥,聂东宋,杨葆生,朱宁..重组人促红细胞生成素纯化工艺的优化[J].生命科学研究,2001,5(2):149-154,6.
