广西植物2003，Vol.23Issue(2)：149-154,148,7.
利用RAPD分子标记对番茄杂交种纯度的鉴定研究
Identification of hybrid purity of tomato (Lyco-persicon esculentum) using RAPD markers
摘要
Abstract
To determine the genetic purity of two commercial tomato (Lycopersicon esculentum) hybrids,Jingdan No.1 and Maofen 802,randomly amplified polymorphic DNA (RAPD) markers were applied to discriminate hybrids from their parental inbred lines. Single primer and two-primer RAPD reactions were assayed. Sixty single primers were applied to discriminate the Jingdan No.1 and Maofen 802 hybrids and their parental inbred lines.Six of them generated polymorphic bands that were different between Jingdan No.1 and its female line. But no informative products produced between Maofen 802 and its parental lines. Jingdan No.1 and Maofen 802 were screened with 14 two-primer reactions. Three two-primer reactions showed polymorphic bands discriminated Jingdan No.1 hybrid from its parental inbred lines and Maofen 802 hybrid from its female inbred lines. The result demonstrate that two-primer RAPD reactions are more effective than the single primer reactions. Six single primer reactions and three two-primer X04-K13, K13-G12, X04-K11 reactions were repeated in National engineering research center for vegetable, Seed purity testing laboratory. 87% paternal-specific markers and hybrid-specific markers in the first experiment, which discriminated Jingdan No.1 hybrid from his parental were generated on another PCR machine, MJ Research PTC-200 and with a different source Taq polymerase purchased from Katara company.关键词
RAPD/单引物/双引物/京丹1号/毛粉802/杂交种纯度Key words
Lycopersicon esculentum/RAPD/two primer/hybrid purity分类
农业科技引用本文复制引用
李丽,E.Klocke..利用RAPD分子标记对番茄杂交种纯度的鉴定研究[J].广西植物,2003,23(2):149-154,148,7.基金项目
中德合作研究项目资助(99-02) （99-02）
