中国全科医学2009，Vol.12Issue(16)：1492-1494,3.
人脐血细胞移植对高血糖模型小鼠的治疗作用
Therapeutic Effect of Human Umbilical Cord Blood Cells on Diabetic Mice
摘要
Abstract
Objective To observe the therapeutic effect of human umbilical cord blood cells transplanted via caudal vein on blood glucose in diabetic mice.Methods Sixty Kunming mice were enrolled,5 of which were chosen as control group,the others given intraperitoneal injection of streptozotocin(STZ) to prepare diabetic mouse models.The prepared models were randomized into groups transportation (n=26) and diabetes (n=12).The isolated umbilical cord blood mononuclearcells were labeled by PKH26 in increased logarithmic phase,and then injected,in 2×106/ml,via caudal vein,into transportation group.The levels of serum glucose and insulin were determined on days 4,14 after injection,and the distribution of PHK26-labeled umbilical cord blood mononuclearcells were observed by flow cytometry and fluorescence microscopy.Results On day 4 after transportation,the serum glucose levels decreased significantly(P<0.05),but insulin levels increased when compared with pre-ransplantation(P<0.05).On day 14,serum glucose returned to pre-transplantation level,and insulin level decreased as compared with level on day 4(P<0.05),not significantly different from control.PKH26 positive cells were found in pancreases,spleen,liver and bone marrow on day 4 after transportation,but not on day 14.The frozen section in these parts supported these results by fluorescence microscopy.Conclusion Human umbilical cord blood cells,which can survive in diabetic mice without immunosuppressive agents ,can reduce blood glucose levels in a certain period of time.关键词
脐血干细胞移植/高血糖小鼠/血糖Key words
Cord blood stem cell tansplantation/Hyperglycemic mice/Blood glucose分类
医药卫生引用本文复制引用
张芳婷,万汇涓,叶静,房家智,尹美珺 ,黄春桥,于洁..人脐血细胞移植对高血糖模型小鼠的治疗作用[J].中国全科医学,2009,12(16):1492-1494,3.基金项目
深圳市科技计划重点项目(200801001) （200801001）
