段绍斌 1于亮 1姚华 2杨明三 1刘郁 1马林杰 1王宏 1张增峰1
作者信息
- 1. 830000,新疆乌鲁木齐,新疆医科大学附属中医医院普外一科
- 2. 公共卫生学院
- 折叠
摘要
Abstract
Objective To explore the hypertonic solution in lung and vascular endothelial cell injury caused by secondary attack of endotoxin after hemorrhagic shock.Methods Sixty-two SD rats were randomly divided into groups sham,lactated Ringer′s solution and 6% hydroxyethyl starch solution resuscitation (RLH group),7.5% sodium chloride solution and 6% hydroxyethyl starch solution resuscitation(HTH group).Hemorrhagic shock models were reproduced in RLH and HTH groups.After resuscitation,the two groups were given endotoxin injection,and sham group given 0.9% sodium chloride solution.Blood and lung tissue specimens were collected at 24 h,48 h,respectively,after injection,to determine the expressions of lung tissue intercellular adhesion molecule -1(ICAM-1) mRNA,lung myeloperoxidase peroxidase(MPO),lung tissue wet/dry weight(W/D) and number of circulating endothelial cells(CEC).Results Intrapulmonary expression of ICAM-1 mRNA,Lung MPO activity,W/D weight ratio and CEC at 24 h were lower in HTH group (37.8±2.5,4.15±0.93 U/g,4.59±0.18,9.1±2.7/0.9 μl,respectively)than in RLH group(46.9±4.1,6.04±1.50 U/g,5.25±0.23,11.8±3.2/0.9 μl,respectively)(P<0.01),and those at hour 48 were lower in HTH group(45.6±6.7,4.62±0.78 U/g,4.84±0.16,13.4±3.5/0.9 μl) than those in RLH group(54.5±6.2,7.59±0.88 U/g,5.31±0.22,16.8±4.2/0.9 μl)(P<0.01).Conclusion Resuscitation with HTH solution can inhibit the expression of ICAM-1mRNA,neutrophil infiltration and accumulation in the lung,related inflammatory reaction during hemorrhagic shock/endotoxin secondary attack,and reduce injury of lung and vascular endothelial cells.关键词
失血性休克/内毒素类/内皮,血管Key words
Hemorrhagic shock/Endotoxins/Endothelium,lymphatic分类
医药卫生
