国际眼科杂志2008，Vol.8Issue(8)：1495-1499,5.
β1整合素过表达抑制角膜上皮细胞凋亡的实验研究
Inhibition of β1-integrin on apoptosis of rabbit corneal epithelial cells
摘要
Abstract
· AIM: To investigate the effect of β1-integrin overexpression on the apoptosis of rabbit corneal epithelial cells and the related mechanism. · METHODS: The plasmid expressing β1-integrin-GFP fusion protein was constructed by polymerase chain reaction (PCR), and this plasmid (β1 group) or the empty vector (mock group) was transfected into rabbit corneal epithelial cells, respectively. The expression of β1-integrin-GFP fusion gene was confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot. The adhesion of transfected cells to extracellular matrix (ECM) proteins was determined by adhesion assay. The apoptosis of rabbit corneal epithelial cells was assayed by Hoechst 33342 staining and DNA ladder. The phosphorylation of mitogen-activated protein (MAP) kinase was examined by Western blot. · RESULTS: Rabbit corneal epithelial cells overexpressing β1 -integrin-GFP fusion gene were successfully established. Compared with mock group, β1-integrin transfection significantly promoted the adhesive of rabbit corneal epithelial cells to ECM proteins such as laminin, fibronectin, collagen Ⅰ and collagen Ⅳ. Β1-integrin overexpression inhibited apoptosis and induced MAP kinase phosphorylation in rabbit corneal epithelial cells (P<0.05).· CONCLUSION: These data suggest that overexpression of β1-integrin confers resistance to apoptosis in rabbit corneal epithelial cells, and MAP kinase pathway may play an important role in this process.关键词
角膜上皮细胞/β1整合素/凋亡/MAP激酶Key words
corneal epithelial cells/β1-integrin/apoptosis/MAP kinase分类
医药卫生引用本文复制引用
王又冬,孙琦,刘冰阳,张劲松..β1整合素过表达抑制角膜上皮细胞凋亡的实验研究[J].国际眼科杂志,2008,8(8):1495-1499,5.基金项目
辽宁省教育厅科学技术研究资助项目(No.20061000)Supported by S＆T Research Project of Liaoning Provincial Department of Education (No.20061000) （No.20061000）
