高等学校化学学报2000，Vol.21Issue(10)：1498-1500,3.
新型pH敏感相分离高分子的制备及其在免疫分析中的应用
Preparation of Novel pH Sensitive Phase Separating Polymer and Its Application in Fluorescence Immunoassay
摘要
Abstract
Poly (N-isopropylacrylamide-methylacrylic acid) [P (NIP-co-MAA) ], a linear water-soluble pH precipitating synthetic polymer, was used as a novel separating carrier for the reactants in immunoassay.The P(NIP-co-MAA) precipitates out of water below a critical pH 5.6 at 37 ℃, and redissolved when the solution pH is above 6. 1. This characteristic of the P(NIP-co-MAA) made it possible to carry out the immunochemical steps of the assay in true solution and then to quickly separate the resulting procucts from the reaction mixtures. The above approach was applied to an assay for the sandwich immunoassay of hepatitis B surface antigen(HBsAg). Sensitivity of this method was close to that of traditional ELISA using same reactants. However, the assay was much faster(assay time decreased from 100－120 min to 45 min). This method has been applied to the determination of the HBsAg levels in human blood serum with satisfactory results. This general technique may also be used for a wide variety of separation processes in addition to immunoassay, in which a specific component is to be isolated for analysis, recovery, or disposal.关键词
pH敏感相分离高分子/免疫分析/乙肝表面抗原Key words
pH sensitive polymer/Phase separation/HBsAg/Fluorescence immunoassay分类
化学化工引用本文复制引用
杨黄浩,朱庆枝,李东辉,陈小兰,丁马太,许金钩..新型pH敏感相分离高分子的制备及其在免疫分析中的应用[J].高等学校化学学报,2000,21(10):1498-1500,3.基金项目
国家自然科学基金(批准号：29775021)和福建省自然科学基金(批准号：B9810004)资助. （批准号：29775021）
