海洋水产研究2000，Vol.21Issue(4)：15,1.
一株产低温碱性蛋白酶嗜冷海洋细菌 YS-9412-130的分离和培养条件研究
Study on isolation and culture condition of one marine bacterium YS-9412-130 producing low-temperature alkaline protease
摘要
Abstract
Sampled from marine shells, after the combined culture with isolating medium and casein medium, the strain YS-9412-130 with high protease activity and high-quality is obtained from over 1 000 samples by the integrated method of testing protease producing proteolytic circle and Folin-phenol assay.The preliminary study of the strain shows that it can only live on organic nitrogen sources; it grows and produces alkaline protease well under the condition of the sanlity near that of natural seawater, temperature (20℃), and pH 7.0～9.0关键词
碱性蛋白酶/海洋细菌/培养条件Key words
Alkaline protease Marine bacterium Culture condition分类
化学化工引用本文复制引用
孙谧,王跃军,张云波,洪义国,阎晓玲,陈毓桢,王春波,刘晓萍..一株产低温碱性蛋白酶嗜冷海洋细菌 YS-9412-130的分离和培养条件研究[J].海洋水产研究,2000,21(4):15,1.基金项目
国家“863”项目（863-819-06-01）资助 （）
