菌物研究2004，Vol.2Issue(3)：1-5,5.
红脐鳞地衣基因组文库的构建
Construction of a Genomic DNA Library of Rhizoplaca chrysoleuca
摘要
Abstract
Lichens are stable symbiotic association comprised of a fungus and one or more photosynthetic microorganisms.Some methods were used to protect the rare and endangered lichens, which included transplanting thalli, separating and culturing fungi, algae and/or cyanobacteria, and constructing the genomic DNA library. As a main means of conservation of rare and endangered lichens, the feasible procedures of constructing the genomic DNA library of lichens were developed. A genomic DNA library of Rhizoplaca chrysoleuca (including both mycobiont and phycobiont) was constructed using the phage Lambda GEM-11 as a vector. The library consisted of 8.5 × 105 clones with an average insert size of about 19 kb. The capacity of this library was about 100 times the equivalent of the sum of haploid genomes of alga and lichen-forming fungus in R. chrysoleuca. Lichens generally grow too slowly for many kinds of laboratory manipulations and for effective conservation of rare and endangered species. Our studies make it possible to do some of those important experimental researches.关键词
红脐鳞/基因组文库/Lambda GEM-11Key words
Rhizoplaca chrysoleuca/Genomic library/Lambda GEM-11分类
农业科技引用本文复制引用
周启明,郭守玉..红脐鳞地衣基因组文库的构建[J].菌物研究,2004,2(3):1-5,5.基金项目
National Natural Science Foundation of China (30230020) （30230020）
Chinese Academy of Sciences (KZ952-J1-112) （KZ952-J1-112）
