中国现代医生2008，Vol.46Issue(1)：1-5,5.
高尿酸血症TNF-α启动子区基因多态性与其代谢表型的关系
The Relationship between the Gene Polymorphism of Tumor Necrosis Factor-αPromoter Region in Hyperuricemia Patients and its Metabolic Phenotype
姚兰兰 1陈新焰 1许凤 1潘杰 1王颜刚1
作者信息
- 1. 青岛大学医学院附属医院内分泌科,青岛,266003
- 折叠
摘要
Abstract
Objective To investigate the correlation between the-308 site,-238 site gene polymorphism of TNF-αa promoter region in hypemricemh and its metabolic phenotype.Methods HUA patients 188,normal people(NHU)51,the-308G/A site single nu-cleofide polymorphism of TNF-a gene promoter region was decided by Polymerase chain reaction-restriction fragment length polymor-phism(PCR-RFLP)method.The level of serum uric acid is detected by uricase.blood glucose by glucose oxidase.Selective inhibition assay for total cholesterol,triglyceride and high-density lipoprotein cholesterol.All these tests were complted within three hours afterextraction.Keep blood samples at-20℃ to detect the concentration of insulin using immunoradiometrie assay.HUA patients 35.NHU 39,the -238G/A site single nucleofide polymorphism of TNF-a gene promoter region was decided by the same method.Results By smdying two single nucleotide polymorphism,the GA+AA frequency of-308 site(23.94%)in HUA was significantly higher than NHU(11.76%)(P=0.0001);the genotype frequencies of GA and GG in-238 site had no significant difference between the two groups(P=0.08).Meanwhile,comparing GA+AA genotype and CG group of-308 site,waist-hip ratio,systolic and diastolic blood pressure,uric acid,triglycerides differences were statistically significant(P=0.05-0.01);the difference in-238 site between the two genotypes waft no significance.Conclusion The results of this study show-308A carriers of TNF-α promoter region in HUA individual is correlated with hyperuficemia and its metabolic phenotype.关键词
高尿酸血症(HUA)/胰岛素/基因多态性/代谢表型/TNF-αKey words
Hyperuricemia/Insulin/Gene polymorphism/Metabolic phenotype/TNF-α分类
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姚兰兰,陈新焰,许凤,潘杰,王颜刚..高尿酸血症TNF-α启动子区基因多态性与其代谢表型的关系[J].中国现代医生,2008,46(1):1-5,5.
