同位素2001，Vol.14Issue(1)：1-5,5.
抗肝癌米托蒽醌免疫毫微粒球的制备及鉴定
Preparation and Determination of Mitoxantrone-loaded Immunonanosphere for Anti-hepatoma
李云春 1张辉敏 1梁正路 1刘晓波 2蔡美英2
作者信息
- 1. 四川大学华西医学中心附属第一医院核医学科,
- 2. 四川大学华西医学中心基础医学院免疫教研室,
- 折叠
摘要
Abstract
After prepared mitoxantrone (DHAQ)-loaded bovine serumalbumin(BSA) nanosphere(DHAQ-BSA-NP) by emulsifying high temperature solidifying method, anti-human hepatoma entire antibody immunonanosphere HAb18-DHAQ-BSA-NP is prepared by modified N-succinimidyl 3-(2-pyridyldithio) propionate(SPDP) technique. The amount of HAb18 on the surface of HAb18-DHAQ-BSA-NP is determined using 125I labelled rabbit antimouse immunoglobulin G(IgG). The results show that HAb18 is bound to the surface of nanosphere DHAQ-BSA-NP and the average amount of HAb18 is 3.5 mg per gram of HAb18-DHAQ-BSA-NP.关键词
米托蒽醌(DHAQ)/抗肝癌/免疫毫微粒球/制备/抗体量分类
医药卫生引用本文复制引用
李云春,张辉敏,梁正路,刘晓波,蔡美英..抗肝癌米托蒽醌免疫毫微粒球的制备及鉴定[J].同位素,2001,14(1):1-5,5.
