核农学报2001，Vol.15Issue(1)：1-5,5.
外源DNA导入普通小麦雄性不育变异体的
花粉母细胞减数分裂观察
MEIOSIS OBSERVATION OF THE STERILE MUTANT AFTER INJECTION OF
EXOGENOUS DNA INTO WHEAT
杨景成 1于元杰 1齐延芳 1沈法富 1刘凤珍1
作者信息
摘要
Abstract
A male sterile mutant was obtained after injection of e xogenous λ DNA into wheat line 814527. Meiosis of pollen mother cells(PMC) of t he mutant and its receptor(line 814527) were observed. The results showed that t h e frequency of chromosomal variation of the sterile line was 18%,and that of the receptor was 0.8%. The main types of variation included univalent, chromosome lagging, chromosome fragment, chromosome bridge, micronucleus, abnormal ditrad a nd tetrad. The fragment of DNA injected into the receptor may influence the norm al genetic process of chromosomes in pollen mother cells, and this may cause var iations of chromosomes. The chromosome variation in meiosis may cause a part of pollen mother cells to abort, but it is not the main cause of abortion.关键词
外源DNA导入/小麦/雄性不育变异/减数分裂分类
农业科技引用本文复制引用
杨景成,于元杰,齐延芳,沈法富,刘凤珍..外源DNA导入普通小麦雄性不育变异体的
花粉母细胞减数分裂观察[J].核农学报,2001,15(1):1-5,5.
